1887

Abstract

Open reading frame (ORF) 9 of the I fragment of the baculovirus multinucleocapsid nuclear polyhedrosis virus (AcMNPV) genome encodes a protein, PK-1, which has strong similarity to serine-threonine protein kinases. The published sequence of the gene contains an error that, when corrected, extends the ORF by 228 nucleotides. Transcription of was first detectable by primer extension at 12 h post-infection, accumulating to high levels during the very late phase of infection. PK-1 produced in rabbit reticulocyte lysates was able to phosphorylate histone HI. Together, our results suggest that ORF 9 encodes a protein kinase that is expressed during the beginning of the late and throughout the very late phases of AcMNPV infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-11-2999
1994-11-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/11/JV0750112999.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-11-2999&mimeType=html&fmt=ahah

References

  1. Barik S., Banerjee A. K. 1992; Sequential phosphorylation of the phosphoprotein of vesicular stomatitis virus by cellular and viral protein kinases is essential for transcription activation. Journal of Virology 66:1109–1118
    [Google Scholar]
  2. Baylis S. A., Banham A. H., Vydelingum S., Dixon L. K., Smith G. L. 1993; African swine fever virus encodes a serine protein kinase which is packaged into virions. Journal of Virology 67:4549–4556
    [Google Scholar]
  3. Bischoff D. S., Slavicek J. M. 1994; Identification and characterization of a protein kinase gene in the Lymantria dispar multi- nucleocapsid nuclear polyhedrosis virus. Journal of Virology 68:1728–1736
    [Google Scholar]
  4. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 35:127–155
    [Google Scholar]
  5. Bradbury E. M., Inglis R. J., Matthews H. R. 1974; Control of cell division by very lysine rich histone (fl) phosphorylation. Nature; London: 247257–261
    [Google Scholar]
  6. Braunagel S. C., Daniel K. D., Reilly L. M., Guarino L. A., Hong T., Summers M. D. 1992; Sequence, genomic organization of the EcoRl-A fragment of Autographa californica nuclear polyhedrosis virus, and identification of a viral-encoded protein resembling the outer capsid protein VP8 of rotavirus. Virology 191:1003–1008
    [Google Scholar]
  7. Chirgwin J. M., Przybla A. E., Mcdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid for sources enriched in ribonuclease. Biochemistry 18:5294–5297
    [Google Scholar]
  8. Colle C. F.III Flowers C. C., O’Callaghan D. J. 1992; Open reading frames encoding a protein kinase, homolog of glycoprotein gX of pseudorabies virus, and a novel glycoprotein map within the unique short segment of equine herpesvirus type 1. Virology 188:545–557
    [Google Scholar]
  9. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  10. Edelman A. M., Blumenthal D. K., Krebs E. G. 1987; Protein serine/threonine kinases. Annual Review of Biochemistry 56:567–613
    [Google Scholar]
  11. Guarino L. A., Summers M. D. 1986; Functional mapping of a Iranr-activating gene required for expression of a baculovirus delayed-early gene. Journal of Virology 60:215–223
    [Google Scholar]
  12. Guarino L. A., Dong W., Xu B., Broussard D. R., Davis R. W., Jarvis D. L. 1992; Baculovirus phosphoprotein pp31 is associated with the virogenic stroma. Journal of Virology 66:7113–7120
    [Google Scholar]
  13. Hanks S. K., Quinn A. M., Hunter T. 1988; The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241:42–52
    [Google Scholar]
  14. Hunter T. 1987; A thousand and one protein kinases. Cell 50:823–829
    [Google Scholar]
  15. Hunter T., Karin M. 1992; The regulation of transcription by phosphorylation. Cell 70:375–387
    [Google Scholar]
  16. Kelly D. C., Lescott T. 1984; Baculovirus replication: phosphorylation of polypeptides synthesized in Trichoplusia ni nuclear polyhedrosis virus-infected cells. Journal of General Virology 65:1183–1191
    [Google Scholar]
  17. Kim D., Weaver R. F. 1993; Transcription mapping and functional analysis of the protein tyrosine/serine phosphatase (PTPase) gene of the Autographa californica nuclear polyhedrosis virus. Virology 195:587–595
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  19. Langan T. A., Gautier J., Lohka M., Hollingsworth R., Moreno S., Nurse P., Maller J., Sclafani R. A. 1989; Mammalian growth-associated HI histone kinase: a homolog of cdc2 + /CDC28 protein kinase controlling mitotic entry in yeast and frog cells. Molecular and Cellular Biology 9:3860–3868
    [Google Scholar]
  20. Leader D. P., Katan M. 1988; Viral aspects of protein phosphorylation. Journal of General Virology 69:1441–1464
    [Google Scholar]
  21. Lin S., Chin W., Broyles S. S. 1992; The vaccinia virus B1R gene product is a serine/threonine protein kinase. Journal of Virology 66:2717–2723
    [Google Scholar]
  22. Maruniak J. E., Summers M. D. 1981; Autographa californica nuclear polyhedrosis virus phosphoproteins and synthesis of intracellular proteins after virus infection. Virology 109:25–34
    [Google Scholar]
  23. Miller L. K., Adang M. J., Browne D. 1983; Protein kinase activity associated with the extracellular and occluded forms of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 46:275–278
    [Google Scholar]
  24. Peter M., Nakagawa J., Doree M., Labbe J. C., Nigg E. A. 1990; In vitro disassembly of the nuclear lamina and M-phase specific phosphorylation of lamins by cdc2 kinase. Cell 61:591–602
    [Google Scholar]
  25. Possee R. D., Sun T. -P., Howard S. C., Ayers M. D., Hill-Perkins M., Gearing K. L. 1991; Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9·4 kbp EcoRl-I and -R (polyhedrin gene) region. Virology 185:229–241
    [Google Scholar]
  26. Prives C. 1990; The replication functions of SV40 T antigen are regulated by phosphorylation. Cell 61:735–738
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sheng Z., Charbonneau H. 1993; The baculovirus Autographa californica encodes a protein tyrosine phosphatase. Journal of Biological Chemistry 268:4728–4733
    [Google Scholar]
  29. Smith G. E., Summers M. D. 1978; Analysis of baculovirus genomes with restriction endonucleases. Virology 89:517–527
    [Google Scholar]
  30. Smith R. F., Smith T. F. 1989; Identification of new protein kinase-related genes in three herpesviruses, herpes simplex virus, varicella-zoster virus, and Epstein-Barr virus. Journal of Virology 63:450–455
    [Google Scholar]
  31. Soderling T. R. 1990; Protein kinases - regulation by autoinhibitory domains. Journal of Biological Chemistry 265:1823–1826
    [Google Scholar]
  32. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin number 1555:
    [Google Scholar]
  33. Taylor S. S. 1989; cAMP-dependent protein kinase-model for an enzyme family. Journal of Biological Chemistry 264:8443–8446
    [Google Scholar]
  34. Vaughn J. L., Goodwin R. H., Thompkins G. J., Mccawley P. 1977; The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In vitro 13:213–217
    [Google Scholar]
  35. Vialard J. E., Richardson C. D. 1993; The 1629-nucleotide open reading frame located downstream of the Autographa californica nuclear polyhedrosis virus polyhedrin gene encodes a nucleocapsid- associated phosphoprotein. Journal of Virology 67:5859–5866
    [Google Scholar]
  36. Whitt M. A., Manning J. S. 1988; A phosphorylated 34-kDa protein and a subpopulation of polyhedrin are thiol-linked to the carbohydrate layer surrounding a baculovirus occlusion body. Virology 163:33–42
    [Google Scholar]
  37. Wilson M. E., Consigli R. A. 1985a; Characterization of a protein kinase activity associated with purified capsids of the granulosis virus infecting Plodia interpunctella. Virology 143:516–525
    [Google Scholar]
  38. Wilson M. E., Consigli R. A. 1985b; Functions of a protein kinase activity associated with purified capsids of the granulosis virus infecting Plodia interpunctella. Virology 143:526–535
    [Google Scholar]
  39. Wu J. Y., Zhou A. -Y., Judd A., Cartwright C. A., Robinson W. S. 1990; The hepatitus B virus-encoded transcriptional transactivator hbx appears to be a novel protein serine/threonine kinase. Cell 63:687–695
    [Google Scholar]
  40. Zemskov E. A., Abramova E. B., Mikhailov V. S. 1992; .Induction of a novel protein kinase in pupae of the silkworm Bombyx mori after infection with nuclear polyhedrosis virus. Journal of General Virology 73:3231–3234
    [Google Scholar]
  41. Zhang G., Stevens R., Leader D. P. 1990; The protein kinase encoded in the short unique region of pseudorabies virus: description of the gene and identification of its product in virions and in infected cells. Journal of General Virology 71:1757–1765
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-11-2999
Loading
/content/journal/jgv/10.1099/0022-1317-75-11-2999
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error