1887

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Volume 75, Issue 11

Association of Serine in Position 1124 of Hantaan Virus Glycoprotein With Virulence in Mice Free

Abstract

Hantaan virus (HV) of the genus causes a fatal disease in suckling mice following intraperitoneal or intracerebral infection. HV cl-1, which was obtained from the 76–118 strain of HV by growth in Vero E6 cells, exhibited high mortality rates in mice whereas mice infected with HV cl-2 survived without any clinical signs. To determine the molecular basis for the marked difference in virulence, we compared the nucleotide sequences of the large (L), medium (M) and small (S) segments of HV cl-1 genome with those of HV cl-2 and found that there was only one predicted amino acid substitution. This amino acid substitution was in position 1124 of the glycoprotein encoded by the M genome segment, in which serine in HV cl-1 was replaced by glycine in HV cl-2. Although there were several nucleotide and amino acid differences between the parental 76–118 strain and HV cl-1, the serine in position 1124 of the glycoprotein was common to the pathogenic parent and the pathogenic mutant. These results suggest that this substitution may be responsible for the virulence of this hantavirus.

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© The Journal of General Virology 1994
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1994-11-01
2024-04-19
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References

  1. Antic D., Kang C. Y., Spik K., Schmaljohn C., Vapalahti O., Vaheri A. 1992; Comparison of the deduced gene products of the L, M and S genome segments of hantaviruses. Virus Research 24:35–46
     [Google Scholar]
  2. Arikawa J., Lapenotiere H. F., Iacono-Connors L., Wang M., Schmaljohn C. S. 1990; Coding properties of the S and the M genome segments of Sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome. Virology 176:114–125
     [Google Scholar]
  3. Chirgwin J. M., Przybyla A. E., Macdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
     [Google Scholar]
  4. Chou P. Y., Fasman G. D. 1978; Empirical predictions of protein conformation. Annual Review of Biochemistry 47:251–276
     [Google Scholar]
  5. Dantas J. R. Jr Okuno Y., Tanishita O., Takahashi Y., Takahashi M., Kurata T., Lee H. W., Yamanishi K. 1987; Viruses of hemorrhagic fever with renal syndrome (FIFRS) grouped by immunoprecipitation and hemagglutination inhibition. Intervirology 27:161–165
     [Google Scholar]
  6. Eisenberg D., Schwarz E., Komaromy M., Wall R. 1984; Analysis of membrane and surface protein sequences with the hydrophobic moment plot. Journal of Molecular Biology 179:125–142
     [Google Scholar]
  7. Endres M. J., Griot C., Gonzales-Scarano F., Nathanson N. 1991; Neuroattenuation of an avirulent bunyavirus variation maps to the L RNA segment. Journal of Virology 65:5465–5470
     [Google Scholar]
  8. Garnier J., Osguthorpe D. J., Robson B. 1978; Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. Journal of Molecular Biology 120:97–120
     [Google Scholar]
  9. Giebel L. B., Stohwasser R., Zöller L., Bautz E. K. F., Darai G. 1989; Determination of the coding capacity of the M genome segment of nephropathia epidemica strain Höllnas B1 by molecular cloning and nucleotide sequence analysis. Virology 172:498–505
     [Google Scholar]
  10. Hjelle B., Jenison S., Torrez-Martinez N., Yamada T., Nolte K., Zumwalt R., Macinnes K., Myers G. 1994; A novel hantavirus associated with an outbreak of fatal respiratory disease in the southwestern United States: evolutinary relationships to known hantaviruses. Journal of Virology 68:592–596
     [Google Scholar]
  11. Isegawa Y., Fujiwara Y., Ohshima A., Fukunaga R., Murakami FI., Yamanishi K., Sokawa Y. 1990; Nucleotide sequence of the M segment of hemorrhagic fever with renal syndrome virus strain B-1. Nucleic Acids Research 18:4936
     [Google Scholar]
  12. Isegawa Y., Sheng J., Sokawa Y., Yamanishi K., Nakagomi O., Ueda S. 1992; Selective amplification of cDNA sequence from total RNA by cassette-ligation mediated polymerase chain reaction (PCR): application to sequencing 6·5 kb genome segment of hantavirus strain B-l. Molecular and Cellular Probes 6:467–475
     [Google Scholar]
  13. Klein P., Kanehisa M., Delisi C. 1985; The detection and classification of membrane-spanning proteins. Biochimica et Bio-physica Acta 815:468–476
     [Google Scholar]
  14. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–307
     [Google Scholar]
  15. McKee K. T. Jr Kim G. R., Green D. E., Peters C. J. 1985; Hantaan virus infection in suckling mice: virologic and pathologic correlates. Journal of Medical Virology 17:107–117
     [Google Scholar]
  16. Naeve C. W., Williams D. 1990; Fatty acids on the A/Japan/305/57 influenza virus haemagglutinin have a role in membrane fusion. EMBO Journal 9:3857–3866
     [Google Scholar]
  17. Nichol S. T., Spiropoulou C. F., Morzunov S., Rollin P. E., Ksiazek T. G., Feldmann H., Sanchez A., Childs J., Zaki S., Peters C. J. 1993; Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science 262:914–917
     [Google Scholar]
  18. Parrington M. A., Lee P. -W., Kang C. Y. 1991; Molecular characterization of the Prospect Hill virus M RNA segment: a comparison with the M RNA segments of other hantaviruses. Journal of General Virology 72:1845–1854
     [Google Scholar]
  19. Rao J. K. M., Argos P. 1986; A conformational preference parameter to predict helices in integral membrane proteins. Bio-chimica et Biophysica Acta 869:197–214
     [Google Scholar]
  20. Rose G. D. 1978; Prediction of chain turns in globular proteins on a hydrophobic basis. Nature; London: 272586–590
     [Google Scholar]
  21. Schmaljohn C. S. 1990; Nucleotide sequence of the L genome segment of Hantaan virus. Nucleic Acids Research 18:6728
     [Google Scholar]
  22. Schmaljohn C. S., Hasty S. E., Dalrymple J. M., Ledue J. W., Lee H. W., Von Bonsdorff C. -H., Brummer-Korvenkontio M., Vaheri A., Tsai T. F., Regnery H. L., Goldgaber D., Lee P. W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
     [Google Scholar]
  23. Schmaljohn C. S., Jennings G., Hay J., Dalrymple J. M. 1986; Coding strategy of the S genome segment of Hantaan virus. Virology 155:633–643
     [Google Scholar]
  24. Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. 1987; Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order. Virology 157:31–39
     [Google Scholar]
  25. Schmaljohn C. S., Arikawa J., Hasty S. E., Rasmussen L., Lee H. W., Lee P. W., Dalrymple J. M. 1988; Conservation of antigenic properties and sequences encoding the envelope proteins of prototype Hantaan virus and two virus isolates from Korean haemorrhagic fever patients. Journal of General Virology 69:1949–1955
     [Google Scholar]
  26. Schmidt M. F. G. 1989; Fatty acylation of proteins. Biochimica et Biophysica Acta 988:411–426
     [Google Scholar]
  27. Spiropoulou C. F., Morzunov S., Feldmann H., Sanchez A., Peters C. J., Nichol S. T. 1994; Genome structure and variability of a virus causing hantavirus pulmonary. Virology 200:715–723
     [Google Scholar]
  28. Stoffel W., Hillen H., Schroder W., Deutzmann W. 1983; The primary structure of bovine myeline lipophilin (proteolipid apoprotein). Hoppe-Seyler’s Zeitschrifte fur Physiologische Chemie 364:1455–1466
     [Google Scholar]
  29. Stone R. 1993; A rogues’ gallery of hantaviruses. Science 262:835
     [Google Scholar]
  30. Tamura M., Asada H., Kondo K., Tanishita O., Kurata T., Yamanishi K. 1989; Pathogenesis of Hantaan virus in mice. Journal of General Virology 70:2897–2906
     [Google Scholar]
  31. Tsai T. F., Baner S., McCormick J. B., Kurata T. 1982; Intracerebral inoculation of suckling mice with Hantaan virus. Lancet ii:503–504
     [Google Scholar]
  32. Veit M., Kretzschmar E., Kuroda K., Garten W., Schmidt M. F. G., Klenk H. -D., Rott R. 1991; Site-specific mutagenesis identifies three cysteine residues in the cytoplasmic tail as acylation sites of influenza virus hemagglutinin. Journal of Virology 65:2491–2500
     [Google Scholar]
  33. Wenzel R. P. 1994; A new hantavirus infection in North America. New England Journal of Medicine 330:1004–1005
     [Google Scholar]
  34. Xiao S. -Y., Leduc J. W., Chu Y. K., Schmaljohn C. S. 1994; Phylogenetic analyses of virus isolates in the genus Hantavirus, family Bunyaviridae . Virology 198:205–217
     [Google Scholar]
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Association of Serine in Position 1124 of Hantaan Virus Glycoprotein With Virulence in Mice
J. Gen. Virol. 75, 3273 (1994); https://doi.org/10.1099/0022-1317-75-11-3273
/content/journal/jgv/10.1099/0022-1317-75-11-3273
/content/journal/jgv/10.1099/0022-1317-75-11-3273
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