1887

Abstract

Two types of Epstein-Barr virus (EBV), EBV-1 and EBV-2, were identified on the basis of DNA sequence divergence in the genes for nuclear proteins EBNA 2, 3a, 3b and 3c. In the present study, we conducted an immunological and genomic analysis in a human immunodeficiency virus (HIV)-infected population to determine the prevalence of the two types, and whether the identified type was stable over years. The EBNA-2 serotyping and genotyping showed that HIV-infected patients were highly infected by EBV-2, and that the dominant strain was mostly retained. However, during a follow-up study, a change in the dominant viral strain was observed in two patients. A first HIV-positive patient (patient A), although having a stable level of anti- EBNA-2A and -2B antibodies, showed a change in the genotype and antigen produced in spontaneously established lymphoblastoid cell lines (LCL). The sequence analysis of LCLs confirmed the emergence of the EBV- 2 type population. A strain from a second HIV-positive patient (patient B) was clearly identified as EBV-2: the genotype from a saliva sample and from sequential LCLs belonged to EBV-2, as well as the antigen produced from LCLs, and serum antibodies. After a 5-year continuous EBV-2 infection, a reactivation of the EBV-1 strain was observed. In both cases, sequence analysis of the EBNA- 2 gene showed, only with EBV-1, the presence of EBV variants related to the B95-8 prototype. Two mutations (at nucleotides 49212 and 49304) were found in both patients A and B, whereas an additional mutation (at nucleotide 49237) was characteristic of the patient A. No mutation relative to the prototype B95-8 strain was observed in a subsequent analysis of this EBNA-2 region from LCLs obtained from two HIV-negative patients predominantly infected by EBV-1. Therefore, we speculate that these mutations may be EBV-1 mutations specifically occurring during HIV infection.

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1994-02-01
2024-03-29
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References

  1. Adldinger H. K., Delius H., Freese U. K., Clarke J., Bornkamm G. W. 1985; A putative transforming gene of Jijoye virus differs from that of Epstein-Barr virus prototype. Virology 141:221–234
    [Google Scholar]
  2. Apolloni A., Moss D., Stumm R., Burrows S., Suhrbier A., Misko I., Schmidt C., Sculley T. 1992; Sequence variation of cytotoxic T cell epitopes in different isolates of Epstein-Barr virus. European Journal of Immunology 22:183–189
    [Google Scholar]
  3. Chen X., Pepper S.Dev Arrand J. R. 1992; Prevalence of the A and B types of Epstein-Barr virus DNA in nasopharyngeal carcinoma biopsies from Southern China. Journal of General Virology 73:463–466
    [Google Scholar]
  4. Dambaugh T., Hennessy K., Chamnankit L., Kieff E. 1984; U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2. Proceedings of the National Academy of Sciences, U.S.A 81:7632–7636
    [Google Scholar]
  5. Kieff E., Liebowitz D. 1990; Epstein-Barr virus and its replication. In Virology, 2nd edn. pp. 1889–1920 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  6. Kunimoto M., Tamura S., Tabata T., Yoshie O. 1992; One-step typing of Epstein-Barr virus by polymerase chain reaction: predominance of type 1 virus in Japan. Journal of General Virology 73:455–461
    [Google Scholar]
  7. Kyaw M. T., Hurren L., Evans L., Moss D. J., Cooper D. A., Benson E., Esmore D., Sculley T. B. 1992; Expression of B- type Epstein-Barr virus in FHV-infected patients and cardiac transplant recipients. AIDS Research and Human Retroviruses 8:1869–1874
    [Google Scholar]
  8. Moss D. J., Misko I. S., Burrows S. R., Burman K., McCarthy R., Sculley T. B. 1988; Cytotoxic T-cell clones discriminate between A- and B-type Epstein-Barr virus transformants. Nature; London: 331719–721
    [Google Scholar]
  9. Mueller-Lantzsch N., Lenoir G. M., Sauter M., Takaki K., Bechet J. M., Kuklik-Roos C., Wunderlich D., Bornkamm G. W. 1985; Identification of the coding region for a second Epstein-Barr virus nuclear antigen (EBNA2) by transfection of cloned DNA fragments. EMBO Journal 4:1805–1811
    [Google Scholar]
  10. Rickinson A. B., Young L. S., Rowe M. 1987; Influence of the Epstein-Barr virus nuclear antigen EBNA 2 on the growth phenotype of virus-transformed B cells. Journal of Virology 61:1310–1317
    [Google Scholar]
  11. Rowe D. T., Clarke J. R. 1989; The type-specific epitopes of the Epstein-Barr virus nuclear antigen 2 are near the carboxy terminus of the protein. Journal of General Virology 70:1217–1229
    [Google Scholar]
  12. Rowe M., Young L. S., Cadwallader K., Petti L., Kieff E., Rickinson A. B. 1989; Distinction between Epstein-Barr virus type A (EBNA 2A) and type B (EBNA 2B) isolates extends to the EBNA 3 family of nuclear proteins. Journal of Virology 63:1031–1039
    [Google Scholar]
  13. Sample J., Young L., Martin B., Chatman T., Kieff E., Rickinson A. B., Kieff E. 1990; Epstein-Barr types 1 and 2 differ in their EBNA-3B, and EBNA-3C genes. Journal of Virology 64:4084–4092
    [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  15. Sculley T. B., Cross S. M., Borrow P., Cooper D. A. 1988; Prevalence of antibodies to Epstein-Barr virus nuclear antigen 2B in persons infected with the human immunodeficiency virus. Journal of Infectious Diseases 158:186–192
    [Google Scholar]
  16. Sculley T. B., Apolloni A., Stumm R., Moss D. J., Mueller-Lantschz N., Misko I. S., Cooper D. A. 1989; Expression of Epstein-Barr virus nuclear antigens 3,4, and 6 are altered in cell lines containing B-type virus. Virology 171:401–408
    [Google Scholar]
  17. Sculley T. B., Apolloni A., Hurren L., Moss D. J., Cooper D. A. 1990; Coinfection with A- and B-type Epstein-Barr virus in human immunodeficiency virus-positive subjects. Journal of Infectious Diseases 162:643–648
    [Google Scholar]
  18. Shu C. H., Chang Y. S., Liang L. C., Liu S. T., Lin C. Z., Chang P. 1992; Distribution of type A and B EBV in normal individuals and patients with head and neck carcinomas in Taiwan. Journal of Virological Methods 38:123–130
    [Google Scholar]
  19. Sixbey J. W., Chesney P. J., Shirley P., Buntin D. M. 1989; Detection of a second widespread strain of Epstein-Barr virus. Lancet ii:761–765
    [Google Scholar]
  20. Sixbey J. W., Shirley P., Sloas M., Raab-Traub N., Israele V. 1991; A transformation-incompetent, nuclear antigen 2-deleted Epstein-Barr virus associated with replicative infection. Journal of Infectious Diseases 163:1008–1015
    [Google Scholar]
  21. Walling D., Edmiston S. N., Sixbey J. W., Abdel-Hamid M., Resnick L., Raab-Traub N. 1992; Coinfection with multiple strains of the Epstein-Barr virus in human immunodeficiency virus-associated hairy leukoplakia. Proceedings of the National Academy of Sciences, U.S.A 89:6560–6564
    [Google Scholar]
  22. Yao Q. Y., Rowe M., Martin B., Young L. S., Rickinson A. B. 1991; The Epstein-Barr virus carrier state: dominance of a single growth-transforming isolate in the blood and in the oropharynx of healthy virus carriers. Journal of General Virology 72:1579–1590
    [Google Scholar]
  23. Zimber U., Adldinger H. K., Lenoir G. M., Vuillaume M., Knebe-Doeberitz M. V., Laux G., Desgrange C., Wittmann P., Freese U. K., Schneider U., Bornkamm G. W. 1986; Geographical prevalence of two types of Epstein-Barr virus. Virology 154:56–66
    [Google Scholar]
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