RT Journal Article SR Electronic(1) A1 Pilling, Andrew A1 Davison, Andrew J. A1 Telford, Elizabeth A. R. A1 Meredith, David M.YR 1994 T1 The equine herpesvirus type 1 glycoprotein homologous to herpes simplex virus type 1 glycoprotein M is a major constituent of the virus particle JF Journal of General Virology, VO 75 IS 2 SP 439 OP 442 DO https://doi.org/10.1099/0022-1317-75-2-439 PB Microbiology Society, SN 1465-2099, AB Glycoprotein 45 is a major envelope glycoprotein of equine herpesvirus type 1. The gene encoding this protein is located between map units 0·615 and 0·636 on the virus genome and evidence has suggested that it is encoded by gene 52, one of four genes within this region. Using PCR we have amplified gene 52 and subsequently cloned it into a mammalian expression vector under the control of the human cytomegalovirus immediate early gene promoter. The gene was expressed in COS-7 cells and its product was detected by immunofluorescence and Western blotting. The results indicate that glycoprotein 45 is encoded by gene 52, and that it is the homologue of herpes simplex virus type 1 glycoprotein M., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-2-439