1887

Abstract

It has been shown previously that the viral glycoprotein VP7 is a major target of cytotoxic T lymphocytes (CTLs) induced by bovine rotavirus RF in C57BL/6 mice. Here we show that these RF-specific CTLs recognize target cells infected with a recombinant vaccinia virus (rW) expressing the SA11 (simian rotavirus) VP6 but not those infected with rWs that express RF VP1, VP2 or VP3 core proteins. After immunization of mice with insect cells infected with recombinant baculoviruses that express the corresponding RF proteins a strong specific CTL response was generated against target cells infected with VP6 rW and VP3 rW, a weak one against the VP2 rW but none against the VP1 rW. K-restricted CTL epitopes were identified on VP6 and VP3 using allele-specific motifs. When peptides corresponding to these epitopes were used to restimulate the spleen cells from RF-immunized mice, CTLs specific for each peptide and its corresponding rW were induced.

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1994-03-01
2024-04-18
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References

  1. Allen H., Wraith D., Pala P., Askonas B. A., Flavell R. A. 1984; Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature; London: 309279–282
    [Google Scholar]
  2. Bennink J. R., Yewdell J. W. 1990; Recombinant vaccinia viruses as vectors for studying T lymphocyte specificity and function. Current Topics in Microbiology and Immunology 163:153–184
    [Google Scholar]
  3. Borrás-Cuesta F., Petit A., Pery A., Fedon Y., Garnier J., Cohen J. 1987; Immunogenicity of synthetic peptides corresponding to regions of the major inner capsid protein of bovine rotavirus (BRV). Annales de l’Institut Pasteur/Virologie 138:437–450
    [Google Scholar]
  4. Buss S., Sette A., Grey H. M. 1987; The interaction between protein-derived immunogenic peptides and la. Immunological Reviews 98:115–141
    [Google Scholar]
  5. Carbone F. R., Bevan M. J. 1990; Class I-restricted processing and presentation of exogenous cell-associated antigen in vivo. Journal of Experimental Medicine 171:377–387
    [Google Scholar]
  6. Cohen J., Lefevre F., Estes M. K., Brémont M. 1984; Cloning of bovine rotavirus (RF strain): nucleotide sequence of the gene coding for the major capsid protein. Virology 138:178–182
    [Google Scholar]
  7. Cohen J., Charpilienne A., Chilmonczyks S., Estes M. K. 1989; Nucleotide sequence of bovine rotavirus gene 1 and expression of the gene product in baculovirus. Virology 171:131–140
    [Google Scholar]
  8. Conner M. E., Matson D. O., Estes M. K. 1993; Rotavirus vaccines and vaccination potential. Current Topics in Microbiology and Immunology in press
    [Google Scholar]
  9. Dharakul T., Labbé M., Cohen J., Bellamy A. R., Street J. E., Mackow E. R., Fiore L., Rott L., Greenberg H. 1991; Immunization with baculovirus-expressed recombinant rotavirus proteins VP1, VP4, VP6, and VP7 induces CD8+ T lymphocytes that mediate clearance of chronic rotavirus infection in SCID mice. Journal of Virology 65:5928–5932
    [Google Scholar]
  10. Drillien R., Spehner D. 1983; Physical mapping of vaccinia virus temperature-sensitive mutations. Virology 131:385–393
    [Google Scholar]
  11. Falk K., Rötzschke O., Stevanović S., Jung G., Rammensee H. 1991; Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules. Nature; London: 351290–296
    [Google Scholar]
  12. Franco M. A., Prieto I., Labbé M., Poncet D., Borràs-Cuesta F., Cohen J. 1993; An immunodominant cytotoxic T cell epitope on the VP7 rotavirus protein overlaps the H2 signal peptide. Journal of General Virology 74:2579–2586
    [Google Scholar]
  13. Hunt F. D., Henderson R. A., Shabanowitz J., Sakaguchi K., Michel H., Sevilir N., Cox A. L., Appella E., Engelhard V. H. 1992; Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science 255:1261–1263
    [Google Scholar]
  14. Kieny M. P., Lathe R., Drillien R., Spehner D., Skory S., Schmitt D., Wiktor T., Koprowski H., Lecocq J. P. 1984; Expression of rabies virus glycoprotein from a recombinant vaccinia virus. Nature; London: 312163–166
    [Google Scholar]
  15. Kitts P. A., Ayres M. D., Possee R. D. 1990; Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Research 18:5667–5672
    [Google Scholar]
  16. Labbé M., Charpilienne A., Crawford S. E., Estes M. K., Cohen J. 1991; Expression of rotavirus VP2 produces empty core like particles. Journal of Virology 65:2946–2952
    [Google Scholar]
  17. L’Haridon R., Scherrer R. 1976; In vitro culture of a rotavirus associated with neonatal calf scours. Annales de Recherches Vétérinaires 7:373–381
    [Google Scholar]
  18. Maeji N. J., Bray A. M., Geysen H. M. 1990; Multi-rod peptide synthesis strategy for T cell determinant analysis. Journal of Immunological Methods 134:23–33
    [Google Scholar]
  19. Martin S., Mercadal C. M., Weir J. P., Rouse B. T. 1993; The proportion of herpes simplex virus-specific cytotoxic T lymphocytes (Tc) that recognizes glycoprotein C varies between individual mice and is dependent on the form of immunization. Viral Immunology 6:21–33
    [Google Scholar]
  20. Matsui S. M., Mackow E. R., Greenberg H. B. 1989; Molecular determinant of rotavirus neutralization and protection. Advances in Virus Research 36:181–214
    [Google Scholar]
  21. Mattion N., Cohen J., Estes M. K. 1993; The rotavirus proteins. In Virus Infections of the GI Tract, 2nd edn. Kapikian A. Edited by New York: Marcel Dekker; in press
    [Google Scholar]
  22. Offit P. A., Dudzik K. I. 1990; Rotavirus-specific cytotoxic T lymphocytes passively protect against gastroenteritis in suckling mice. Journal of Virology 64:6325–6328
    [Google Scholar]
  23. Offit P. A., Boyle D. B., Both G. W., Hill N. L., Svoboda Y. M., Cunningham S. L., Jenkins R. J., Mccrae M. A. 1991; Outer capsid glycoprotein VP7 is recognized by cross-reactive, rotavirus specific, cytotoxic T lymphocytes. Virology 184:563–568
    [Google Scholar]
  24. Openshaw P. J. M., Anderson K., Wertz G. W., Askonas B. A. 1990; The 22,000-kilodalton protein of respiratory syncytial virus is a major target for Kd-restricted cytotoxic T lymphocytes from mice primed by infection. Journal of Virology 64:1683–1689
    [Google Scholar]
  25. Pala P., Townsend A. R. M., Askonas B. A. 1986; Viral recognition by influenza A virus cross-reactive cytotoxic T (Tc) cells: the proportion of Tc cells that recognize nucleoprotein varies between individual mice. European Journal of Immunology 16:193–198
    [Google Scholar]
  26. Poncet D., Corthier G., Charpilienne A., Cohen J. 1990; A recombinant vaccinia virus expressing the major capsid protein of simian rotavirus-induced anti-rotavirus antibodies. Virus Research 15:267–274
    [Google Scholar]
  27. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin1555
    [Google Scholar]
  28. Tosser G., Labbé M., Brémont M., Cohen J. 1992; Expression of the major capsid protein VP6 of group C rotavirus and synthesis of chimeric single-shelled particles by using recombinant baculo-viruses. Journal of Virology 66:5825–5831
    [Google Scholar]
  29. Townsend A. R. M., Mcmichael A. J., Carter N. P., Huddleston J. A., Brownlee G. G. 1984; Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells. Cell 39:13–25
    [Google Scholar]
  30. Wei M. L., Cresswell P. 1992; HLA-A2 molecules in an antigenprocessing mutant cells contain signal sequence-derived peptides. Nature; London: 356443–446
    [Google Scholar]
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