RT Journal Article SR Electronic(1) A1 Willcocks, M. M. A1 Brown, T. D. K. A1 Madeley, C. R. A1 Carter, M. J.YR 1994 T1 The complete sequence of a human astrovirus JF Journal of General Virology, VO 75 IS 7 SP 1785 OP 1788 DO https://doi.org/10.1099/0022-1317-75-7-1785 PB Microbiology Society, SN 1465-2099, AB We have determined the complete genomic sequence of human astrovirus serotype 1 isolated in Newcastle upon Tyne. The genome is 6813 nucleotides long and contains three sequential open reading frames (ORFs). The two closest to the 5′ end are linked by a ribosomal frameshifting motif and contain sequence motifs indicative of non-structural virus proteins: serine protease and RNA-dependent RNA polymerase. A nuclear addressing sequence is also located here. The 3′ ORF encodes the virion structural polypeptides as a polyprotein precursor. This genomic organization resembles that of the plant virus family Luteoviridae. Nucleotide sequence data described in this paper appear in the EMBL database under accession no. Z25771., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-7-1785