1887

Abstract

Two filamentous phages of pv. and pv. were isolated and designated Xv and Xo, respectively. They were similar to other filamentous phages of in (i) shape, (ii) restrictive host specificity, (iii) high stability, (iv) an ssDNA genome, (v) a dsDNA as the replicative form (RF), (vi) propagation without lysis of host cells and (vii) ability to integrate into the host chromosome. These phages showed sequence homology to filamentous phage Lf of pv. Xv was inactivated by antisera against Xv, Xo and Lf, whereas o and Lf were inactivated only by their respective antisera and the anti-Xv serum. Both the single-stranded phage DNAs and the RF DNAs of Xv, Xo and Lf were able to transfect pv. pv. and pv. Physical maps of Xv and Xo were constructed for the RF DNAs. Genome sizes were estimated, based on mapping data, to be 6·8 kb for Xv and 7·6 kb for Xo, larger than that of the ;Lf genome (6·0 kb). The difference in genome sizes appeared to result from insertions of large DNA fragments. These fragments and the regions mediating integration were localized in the physical maps.

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1994-09-01
2024-03-29
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References

  1. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction method for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. Bradbury J. F. 1984; Genus II. Xanthomonas . In Bergey’s Manual of Systematic Bacteriology 1 pp 199–210 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Chang R.-Y. 1989 Integration of cloned DNA into chromosome of Xanthomonas campestris mediated by the filamentous phage 𝜙Lf DNA M. S. thesis National Chung Hsing University:
    [Google Scholar]
  4. Dai H., Chiang K.-S., Kuo T.-T. 1980; Characterization of a new filamentous phage Cf from Xanthomonas citri . Journal of General Virology 46:277–289
    [Google Scholar]
  5. Dai H., Tsay S.-H., Kuo T.-T., Lin Y.-H., Wu W.-C. 1987; Neolysogenization of Xanthomonas campestris pv. citri infected with filamentous phage Cfl6. Virology 156:313–320
    [Google Scholar]
  6. Dai H., Liao H.-J., Chiang K.-S. 1988; Differential stability of filamentous phage genomes in Xanthomonas campestris pv. citri . Microbios 56:157–167
    [Google Scholar]
  7. Eisenstark A. 1967; Bacteriophage techniques. In Methods in Virology 1 pp 449–524 Maramorosch K., Koprowski H. Edited by New York: Academic Press;
    [Google Scholar]
  8. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2
    [Google Scholar]
  9. Frangione B., Nakashima Y., Konigsberg W., Wiseman R. L. 1978; The amino acid sequence of the major coat protein subunit of the filamentous virus Xf. FEBS Letters 96:381–384
    [Google Scholar]
  10. Fu J.-F., Chang R.-Y., Tseng Y.-H. 1992; Construction of stable lactose-utilizing Xanthomonas campestris by chromosomal integration of cloned lac genes using filamentous phage 𝅹Lf DNA. Applied Microbiology and Biotechnology 37:225–229
    [Google Scholar]
  11. Kamiunten H., Wakimoto S. 1979; Biological properties of the filamentous phages released from Xanthomonas oryzae isolates. Annals of the Phytopathology Society of Japan 45:174–181
    [Google Scholar]
  12. Kuo T.-T., Huang T.-C., Chow T.-Y. 1969; A filamentous bacteriophage from Xanthomonas oryzae . Virology 39:548–555
    [Google Scholar]
  13. Kuo T.-T., Tan M.-S., Sue M.-T., Yang M.-K. 1991; Complete nucleotide sequence of filamentous phage Cflc from Xanthomonas campestris pv. citri . Nucleic Acids Research 19:2498
    [Google Scholar]
  14. Ma M., Burkholder J. K., Webb R. E., Hsu H. T. 1984; Plastic- bead ELISA: an inexpensive epidemiological tool for detecting gypsy moth (Lepidoptera : Lymantriidae ) nuclear polyhedrosis virus. Journal of Economic Entomology 77:537–540
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Miller J. H. 1972 Experiments in Molecular Genetics New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Minsavage G. V., Dahlbeck D., Whalen M. C., Kearney B., Bonas U., Staskawicz B. J., Stall R. E. 1990; Gene-for-gene relationships specifying disease resistance in Xanthomonas campestris pv. vesicatoria- pepper interactions. Molecular Plant-Microbe Interactions 3:41–17
    [Google Scholar]
  18. Ou S. H. 1972 Rice Diseases Kew: Commonwealth Mycological Institute;
    [Google Scholar]
  19. Pai M.-S. 1989 Studies on the insertion of filamentous phage DNA into the host chromosome M. S. thesis National Taiwan University:
    [Google Scholar]
  20. Sandford P. A., Baird J. 1983; Industrial utilization of polysaccharides. In The Polysaccharides 2 pp 411–190 Aspinall G. O. Edited by New York: Academic Press;
    [Google Scholar]
  21. Slodki M. E., Cadmus M. C. 1978; Production of microbial polysaccharide. Advances in Applied Microbiology 23:19–54
    [Google Scholar]
  22. Swings J., Vandenmooter M., Vauterin L., Hoste M., Gillis M., Mew T. W., Kersters K. 1990; Reclassification of the causal agents of bacterial blight (Xanthomonas campestris pv. oryzae) and bacterial leaf streak (Xanthomonas campestris pv. oryzicola) of rice as pathovars of Xanthomonas oryzae (ex Ishiyama 1922) sp. nov., nom. rev. International Journal of Systematic Bacteriology 40:309–311
    [Google Scholar]
  23. Tseng Y.-H., Lo M.-C., Lin K.-C, Chang R.-Y. 1990; Characterization of filamentous bacteriophage 𝜙Lf from Xanthomonas campestris pv. campestris . Journal of General Virology 71:1881–1884
    [Google Scholar]
  24. Vauterin L., Swings J., Kersters K., Gillis M., Mew T. W., Schroth M. N., Palleroni N. J., Hildebrand D. C., Stead D. E., Civerolo E. L., Hayward A. C., Maraite H., Stall R. E., Vidaver A. K., Bradbury J. F. 1990; Towards an improved taxonomy of Xanthomonas . International Journal of Systematic Bacteriology 40:312–316
    [Google Scholar]
  25. Vieira J., Messing J. 1991; New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100:189–194
    [Google Scholar]
  26. Wang T.-W., Tseng Y.-H. 1992; Electrotransformation of Xanthomonas campestris by RF DNA of filamentous phage ^Lf. Letters in Applied Microbiology 14:65–68
    [Google Scholar]
  27. Wen F.-S. 1992 Genomic organization of filamentous bacteriophage 𝅹Lf of Xanthomonas campestris pv. campestris Ph. D. thesis National Chung Hsing University:
    [Google Scholar]
  28. William P. H. 1980; Black rot: a continuing threat to world crucifers. Plant Disease 64:736–742
    [Google Scholar]
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