Characterization of two novel filamentous phages of Xanthomonas
Lin, Nien-Tsung and You, Bih-Yuh and Huang, Chang-Yi and Kuo, Chung-Wen and Wen, Fu-Shyan and Yang, Jui-Sen and Tseng, Yi-Hsiung,, 75, 2543-2547 (1994),
doi = https://doi.org/10.1099/0022-1317-75-9-2543,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Two filamentous phages of Xanthomonas campestris pv. vesicatoria and Xanthomonas oryzae pv. oryzae were isolated and designated ϕXv and ϕXo, respectively. They were similar to other filamentous phages of Xanthomonas in (i) shape, (ii) restrictive host specificity, (iii) high stability, (iv) an ssDNA genome, (v) a dsDNA as the replicative form (RF), (vi) propagation without lysis of host cells and (vii) ability to integrate into the host chromosome. These phages showed sequence homology to filamentous phage ϕLf of X. c. pv. campestris. ϕXv was inactivated by antisera against ϕXv, ϕXo and ϕLf, whereas ϕ Xo and ϕLf were inactivated only by their respective antisera and the anti-ϕXv serum. Both the single-stranded phage DNAs and the RF DNAs of ϕXv, ϕXo and ϕLf were able to transfect X. c. pv. vesicatoria, X. o. pv. oryzae and X. c. pv. campestris. Physical maps of ϕXv and ϕXo were constructed for the RF DNAs. Genome sizes were estimated, based on mapping data, to be 6·8 kb for ϕXv and 7·6 kb for ϕXo, larger than that of the ;ϕLf genome (6·0 kb). The difference in genome sizes appeared to result from insertions of large DNA fragments. These fragments and the regions mediating integration were localized in the physical maps.,
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