f Early replication block of human immunodeficiency virus type 1 in monkey cells
- Authors: Riri Shibata*, Hiroyuki Sakai, Meiko Kawamura, Kenzo Tokunaga, Akio Adachi†
*Author for correspondence. Fax +81 75 751 3995. e-mail firstname.lastname@example.org
- J. Gen. Virol., November 1995 76: 2723-2730, doi: 10.1099/0022-1317-76-11-2723
- Subject: Animal
- Published Online:
The genetic and functional basis of the replication-defective nature of human immunodeficiency virus type 1 (HIV-1) in monkey cells was studied. By the generation and characterization of chimeras between HIV-1 and simian immunodeficiency virus, the sequence encompassing the 3′ half of the long terminal repeat, gag and pol genes of HIV-1 was found to be responsible for the growth restriction. Early and late phases of HIV-1 replication in monkey cells were analysed in detail using several assay systems: transfection/coculture, trans-complementation between various proviral clones carrying the CAT gene and effector clones and evaluation of transcription and reverse transcription. All the data were consistent with the notion that HIV-1 replication is blocked at a very early stage(s) such as uncoating and/or reverse transcription in monkey cells.
Author for correspondence. Fax +81 75 751 3995. e-mail email@example.com
Present address: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
© Society for General Microbiology 1995 | Published by the Microbiology Society
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