f Genetic and antigenic properties of Dobrava virus: a unique member of the Hantavirus genus, family Bunyaviridae
- Authors: Tatjana Avsic-Zupanc*, Alison Toney, Kevin Anderson, Yong-Kyu Chu†, Connie Schmaljohn‡
*Author for correspondence. Fax +1 301 619 2439. e-mail email@example.com
- J. Gen. Virol., November 1995 76: 2801-2808, doi: 10.1099/0022-1317-76-11-2801
- Subject: Animal
- Published Online:
We examined the genetic and antigenic properties of Dobrava (DOB) virus, a hantavirus associated with severe haemorrhagic fever with renal syndrome in Europe. Cloning and sequence analyses revealed the DOB M segment to consist of 3644 nucleotides, with a coding capacity of 1134 amino acids in the virus complementary-sense RNA (cRNA). Seven potential asparagine-linked glycosylation sites were identified in the M segment gene product, one in the G2 and six in the G1 coding regions. The S segment is 1667 nucleotides long, and has a single ORF in the cRNA capable of encoding a protein of 428 amino acids. Phylogenetic comparisons of the M and S segments of DOB virus to those of other hantaviruses indicated that DOB virus is similar to, but clearly distinct from Hantaan (HTN) and Seoul (SEO) viruses. Certain G2-specific, but not G1-specific monoclonal antibodies to HTN virus reacted to the same titre with DOB and homologous viral antigen. Plaque-reduction neutralization tests indicated that, of the sera tested, only antisera to SEO virus were able to neutralize DOB virus to a titre greater than 1:10; however, this neutralization titre was eightfold lower than that observed with homologous SEO virus. The data reported here confirm that DOB virus is a unique species in the Hantavirus genus, family Bunyaviridae.
Author for correspondence. Fax +1 301 619 2439. e-mail firstname.lastname@example.org
Present address: Institute of Microbiology, Medical Faculty of Ljubljana, Zalosska 4, 61105 Ljubljana, Slovenia.
Present address: ASAN Institute for Life Sciences, 388-1 Poongnap-Dong, Songpa-Ku, Seoul 138-040, Korea.
© Society for General Microbiology 1995 | Published by the Microbiology Society
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