f Mutational analysis of influenza virus promoter elements in vivo
- Authors: Gabriele Neumann, Gerd Hobom*
*Author for correspondence. Fax +49 641 702 9659.
- First Published Online: 01 July 1995, Journal of General Virology 76: 1709-1717, doi: 10.1099/0022-1317-76-7-1709
- Subject: Animal
- Issue Published:
RNA polymerase I transcription in vivo in transiently DNA-transfected cells has been used to express influenza virus vRNA molecules coding for chloramphenicol acetyltransferase (CAT) in an antisense orientation. Influenza virus superinfection provided viral RNA polymerase and other proteins required for transcriptional conversion of minus-strand vRNA into plus-strand viral mRNA molecules expressing CAT activity. This system has been used for analysis of the vRNA sequences which cooperatively constitute the vRNA promoter structure via nucleotide exchanges as well as deletions and insertions of both terminal segments. Several mutants caused greatly enhanced expression over wild-type levels, which was transmitted during serial passage of progeny virus. The data obtained for the mutations in various promoter elements support a model implicating double-stranded vRNA promoter structures in binding of viral polymerase, and in consecutive steps during initiation of RNA synthesis.
Author for correspondence. Fax +49 641 702 9659.
© Society for General Microbiology 1995 | Published by the Microbiology Society
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