Isolation and characterization of Tula virus, a distinct serotype in the genus Hantavirus, family Bunyaviridae Vapalahti, Olli and Lundkvist, Åke and Kukkonen, Sami K. J. and Cheng, Ying and Gilljam, Mari and Kanerva, Mari and Manni, Tytti and Pejcoch, Milan and Niemimaa, Jukka and Kaikusalo, Asko and Henttonen, Heikki and Vaheri, Antti and Plyusnin, Alexander,, 77, 3063-3067 (1996), doi = https://doi.org/10.1099/0022-1317-77-12-3063, publicationName = Microbiology Society, issn = 0022-1317, abstract= A Vero E6 cell culture isolate of Tula virus (TUL), a hantavirus first detected in European common voles (Microtus arvalis and M. rossiaemeridionalis) by RT-PCR was obtained after initial passaging of TUL-infected vole lung samples in laboratory-colonized M. arvalis. TUL was defined as a classical serotype by a cross-focus-reduction neutralization test (FRNT) and was also shown to be distinct from other hantaviruses by haemagglutination inhibition assay. The sequences of S, M and partial L genome segments of the isolate were determined: the S segment was 99.9% identical to the original rodent-derived sequence. Serological evidence for a previous TUL infection was obtained from the serum of a blood donor living near a TUL focus in Moravia, Czech Republic, showing at least a 16-fold higher FRNT titre to TUL as compared to Puumala or other hantaviruses., language=, type=