Beet necrotic yellow vein virus 42 kDa triple gene block protein binds nucleic acid in vitro
Bleykasten, Claudine and Gilmer, D. and Guilley, H. and Richards, K. E. and Jonard, G.,, 77, 889-897 (1996),
doi = https://doi.org/10.1099/0022-1317-77-5-889,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= The triple gene block (TGB) of beet necrotic yellow vein virus RNA 2 is required for cell-to-cell movement of the virus RNA. The protein P42 encoded by the 5′-proximal gene of the TGB has consensus sequence motifs characteristic of an ATP/GTP-dependent helicase. P42 was over-expressed in Escherichia coli and shown to bind both single- and double-stranded RNA and DNA by Northwestern blotting. Site-directed mutagenesis located the nucleic acid-binding domain to the N-terminal 24 amino acids of the protein and a point mutation or deletions in the region of P42 containing the helicase consensus sequences did not affect nucleic acid-binding activity of the immobilized protein. Electrophoretic mobility-shift assays revealed that P42 also binds nucleic acids in solution and that deletion of the N-terminal region inhibits this binding. Mutations in both the N-terminal nucleic acid-binding domain and the helicase domain blocked infection of leaves, indicating that both regions of P42 are important for its activity in vivo.,
language=,
type=