f Large RNA segment of Dugbe nairovirus encodes the putative RNA polymerase
- Authors: Anthony C. Marriott, Patricia A. Nuttall
- First Published Online: 01 August 1996, Journal of General Virology 77: 1775-1780, doi: 10.1099/0022-1317-77-8-1775
- Subject: Animal - RNA viruses
- Issue Published:
The nucleotide sequence of the large (L) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) was determined, completing the first entire genome sequence of a nairovirus. The L segment comprised 12255 nucleotides, making a total genome size of 18855 nucleotides, and the ends showed identity with the ends of the medium (M) and small (S) genomic segments. A single open reading frame (ORF) was present in the viral complementary strand, sufficient to encode a protein of 459 kDa. The predicted protein sequence showed the core polymerase motifs characteristic of the RNA-dependent RNA polymerases of segmented negative-stranded viruses. Comparison of the conserved motifs with the corresponding region of other segmented negative-strand viruses showed a closer relationship between nairoviruses and phleboviruses than with other Bunyaviridae or with other virus families. However, the core polymerase was the only function that could be assigned to a region of the DUG L gene.
© Society for General Microbiology Ltd 1996 | Published by the Microbiology Society
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