1887

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Volume 77, Issue 8

Protease-activated lymphoid cell and hepatocyte recognition site in the preS1 domain of the large woodchuck hepatitis virus envelope protein Free

Abstract

A site capable of strictly host- and cell type-specific recognition was identified in the preS1 domain of woodchuck hepatitis virus (WHV) through the use of antipeptide antisera generated against the extreme N-terminal fragment of the large virus envelope protein. The crucial determinant of this binding site was mapped to amino acids 10–13. Although a synthetic analogue of the site was highly immunogenic, natural WHV envelope did not display the site activity unless it was modified by proteolysis or acidic pH treatment, indicating an internal location of the determinant in viral envelope. Synthetic peptides encompassing the sequence of this site bound woodchuck lymphoid cells and hepatocytes in a species-restricted manner which followed characteristics of a specific ligand-receptor interaction, although their ability to interact with lymphoid cells was considerably greater than that for hepatocytes. In WHV-infected animals, a netural antibody to the identified cryptic cell-binding site arose independently of that directed against epitopes of unmodified virus envelope and its appearance constituted the earliest immunovirological indicator of virus invasion. Our results demonstrated that the preS1 domain of the large WHV envelope protein is endowed with the species- and cell type-specific recognition site which is protected against antibody surveillance by the natural tertiary structure of the protein and we suggest that proteolytic cleavage is required to induce the binding activity.

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© Journal of General Virology 1996
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1996-08-01
2024-04-23
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References

  1. Budkowska A., Quan C., Groh F., Bedossa P., Dubreuil P., Bouvet J. P., Pillot J. 1993; Hepatitis B virus (HBV) binding factor in human plasma: candidate for a soluble form of hepatocyte HBV receptor. Journal of Virology 76:4316–4322
     [Google Scholar]
  2. Chemin I., Baginski I., Vermot-Desroches C., Hantz O., Jacquet C., Rigal D., Trepo C. 1992; Demonstration of woodchuck hepatitis virus infection of peripheral blood mononuclear cells by flow cytometry and polymerase chain reaction. Journal of General Virology 73:123–129
     [Google Scholar]
  3. Colucci G., Lyons P., Beazer Y., Waksal S. D. 1988; Production of hepatitis B-virus-infected human B-cell hybridomas: transmission of the viral genome to normal lymphocytes in cocultures. Virology 164:238–244
     [Google Scholar]
  4. Dash S., Rao K. V. S., Panda S. K. 1992; Receptor for pre-S1 (21–47) component of hepatitis B virus on the liver cell: role in virus cell interaction. Journal of Medical Virology 37:116–121
     [Google Scholar]
  5. Franco A., Paroli M., Testa U., Benvenutto R., Peschle C., Balsano F., Barnaba V. 1992; Transferrin receptor mediates uptake and presentation of hepatitis B envelope antigen by T lymphocytes. Journal of Experimental Medicine 175:1195–1205
     [Google Scholar]
  6. Galibert F., Chen T.-N., Mandart E. 1982; Nucleotide sequence of a cloned woodchuck hepatitis virus genome:comparison with the hepatitis B virus sequence. Journal of Virology 41:51–65
     [Google Scholar]
  7. Gerlich W. H., Lu X., Heermann K. H. 1993; Studies on the attachment and penetration of hepatitis B virus. Journal of Hepatology 17: supplementum 3S10–S14
     [Google Scholar]
  8. Gotoh B., Ogasawara T., Toyoda T., Inocencio N. M., Hamaguchi M., Nagai Y. 1990; An endoprotease homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo. EMBO Journal 9:4189–4190
     [Google Scholar]
  9. Hallenberger S., Bosch V., Angliker H., Shaw E., Klenk H.-D., Garten W. 1992; Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160. Nature 360:358–361
     [Google Scholar]
  10. Heermann K. H., Gerlich W. H. 1991; Surface proteins of hepatitis B viruses. In Molecular biology of the hepatitis B viruses pp 110–143 Edited by McLachlan A. L. Boca Raton, FL: CRC Press;
     [Google Scholar]
  11. Jin Y.-M., Pardoe I. U., Burness A. T. H., Michalak T. I. 1994; Identification and characterization of the cell surface 70-kilodalton sialoglycoprotein(s) as a candidate receptor for encephalomyocarditis virus on human nucleated cells. Journal of Virology 68:7308–7319
     [Google Scholar]
  12. Laure F., Zagury D., Saimot A. G., Gallo R. C., Hahn B. H., Brechot C. 1985; Hepatitis B virus DNA sequences in lymphoid cells from patients with AIDS-related complex. Science 229:561–563
     [Google Scholar]
  13. Klenk H.-D., Garten W. 1994; Host cell proteases controlling virus pathogenicity. Trends in Microbiology 2:39–43
     [Google Scholar]
  14. Korba B. E., Wells F., Tennant B. C., Cote P., Gerin J. L. 1987; Lymphoid cells in the spleens of woodchuck hepatitis virus-infected woodchucks are a site of active viral replication. Journal of Virology 61:1318–1324
     [Google Scholar]
  15. Korba B. E., Cote P., Gerin J. L. 1988; Mitogen-stimulated replication of woodchuck hepatitis virus by lipopolysaccharide-stimu-lated peripheral blood lymphocytes. Science 241:1213–1216
     [Google Scholar]
  16. Mason A., Yoffe B., Noonan C., Mearns M., Campbell C., Kelley A., Perrillo R. B. 1992; Hepatitis B virus DNA in peripheral-blood mononuclear cells in chronic hepatitis B after HBsAg clearance. Hepatology 16:36–41
     [Google Scholar]
  17. Michalak T. I., Churchill N. D. 1988; Interaction of woodchuck virus surface antigen with hepatocyte plasma membrane in woodchuck chronic hepatitis. Hepatology 8:499–506
     [Google Scholar]
  18. Michalak T. I., Lin B. 1994; Molecular species of hepadnavirus core and envelope polypeptides in hepatocyte plasma membrane of woodchucks with acute and chronic viral hepatitis. Hepatology 20:275–286
     [Google Scholar]
  19. Michalak T. I., Lin B., Churchill N. D., Dzwonkowski P., Desousa J. R. B. 1990; Hepadnavirus nucleocapsid and surface antigens and the antigen-specific antibodies associated with hepatocyte plasma membranes in experimental woodchuck acute hepatitis. Laboratory Investigation 62:680–689
     [Google Scholar]
  20. Michalak T. I., Desousa J. R. B., Churchill N. D., Rangraju N. S., Ananthanarayanan V. S. 1991; Host and organ recognition site within preS1-encoded sequence of woodchuck hepatitis virus. In Viral hepatitis and liver disease pp 262–266 Edited by Hollinger F. B., Lemon S. M., Margolis H. Baltimore: Williams and Wilkins;
     [Google Scholar]
  21. Michalak T. I., Pasquinelli C., Guilhot S., Chisari F. V. 1994a; Hepatitis B virus persistence after recovery from acute viral hepatitis. Journal of Clinical Investigation 93:230–239
     [Google Scholar]
  22. Michalak T. I., Pardoe I. U., Deng L. T. 1994b; Persistent carriage of WHV in woodchucks convalescent from an acute episode of viral hepatitis. Hepatology 20:303A
     [Google Scholar]
  23. Michalak T. I., Churchill N. D., Codner D., Drover S., Marshall W. H. 1995; Identification of woodchuck class I MHC antigens using monoclonal antibodies. Tissue Antigens 45:333–342
     [Google Scholar]
  24. Neurath A. R., Kent S. B. H., Strick N., Parker P. 1986; Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46:426–436
     [Google Scholar]
  25. Neurath A. R., Strick N., Sporoul P., Ralph H. E., Vaiinsky J. 1990; Detection of receptors for hepatitis B virus on cells of extrahepatic origin. Virology 176:448–457
     [Google Scholar]
  26. Neurath A. R., Strick N., Sprout P. 1992; Search for hepatitis B virus cell receptors reveals binding sites for interleukin 6 on the virus envelope protein. Journal of Experimental Medicine 175:461–469
     [Google Scholar]
  27. Noonan C. B., Yoffe B., Mansell P., Melnick J., Hollinger B. 1986; Extrachromosomal sequences of hepatitis B virus DNA in peripheral mononuclear cells of acquired immune deficiency syndrome patients. Proceedings of the National Academy of Sciences, USA 83:5698–5702
     [Google Scholar]
  28. Ogston C. W., Schechter E. M., Humes C. A., Pranikoff M. B. 1989; Extrahepatic replication of woodchuck hepatitis virus in chronic infection. Virology 169:9–14
     [Google Scholar]
  29. Pardoe I. U., Michalak T. I. 1995; Detection of hepatitis B and woodchuck hepatitis viral DNA in plasma and mononuclear cells from heparinized blood by the polymerase chain reaction. Journal of Virological Methods 51:277–288
     [Google Scholar]
  30. Pasquinelli C., Melegari M., Villa E., Scaglioni P. P., Seidenari N., Mongiardo N., De Rienzo B., Manenti F. 1990; Hepatitis B virus infection of peripheral blood mononuclear cells is common in acute and chronic hepatitis. Journal of Medical Virology 31:135–140
     [Google Scholar]
  31. Persing D. H., Varmus H. E., Ganem D. 1986; Antibodies to preS and X determinants arise during natural infection with ground squirrel hepatitis virus. Journal of Virology 60:177–184
     [Google Scholar]
  32. Petit M.-A., Capel F., Dubanchet S., Mabit H. 1992; PreS1-specific binding proteins as potential receptors for hepatitis B virus in human hepatocytes. Virology 187:211–222
     [Google Scholar]
  33. Pontisso P., Petit M.-A., Bankowski M. J., Peeples M. E. 1989; Human liver plasma membranes contain receptors for the hepatitis B virus pre-Sl region and, via polymerized human serum albumin, for the pre-S2 region. Journal of Virology 63:1981–1988
     [Google Scholar]
  34. Pontisso P., Ruvoletto M. G., Tiribelli C., Gerlich W. H., Ruol A., Alberti A. 1992; The pre-S1 domain of hepatitis B virus and IgA cross-react in their binding to the hepatocyte surface. Journal of General Virology 73:2041–2045
     [Google Scholar]
  35. Schaeffer E., Snyder R. L., Sninsky J. J. 1986; Identification and localization of pre-s-encoded polypeptides from woodchuck and ground squirrel hepatitis viruses. Journal of Virology 57:173–182
     [Google Scholar]
  36. Treichel U., Meyer zum Buschenfelde K. H., Stockert R. J., Poralla T., Gerken G. 1994; The asialoglycoprotein receptor mediates hepatic binding and uptake of natural hepatitis B virus particles derived from viraemic carriers. Journal of General Virology 75:3021–3029
     [Google Scholar]
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Protease-activated lymphoid cell and hepatocyte recognition site in the preS1 domain of the large woodchuck hepatitis virus envelope protein
J. Gen. Virol. 77, 1837 (1996); https://doi.org/10.1099/0022-1317-77-8-1837
/content/journal/jgv/10.1099/0022-1317-77-8-1837
/content/journal/jgv/10.1099/0022-1317-77-8-1837
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