f Quantification of Human Herpesvirus 6 in Immunocompetent Persons and Post-mortem Tissues from AIDS Patients by PCR
- Authors: Duncan A. Clark, Mounir Ait-Khaled, Anne C. Wheeler, I. Michael Kidd, James E. McLaughlin, Margaret A. Johnson, Paul D. Griffiths, Vincent C. Emery
- Author for correspondence: Vincent C. Eme W. Fax +44 171 830 2854, e-mail firstname.lastname@example.org
- J. Gen. Virol., September 1996 77: 2271-2275, doi: 10.1099/0022-1317-77-9-2271
- Subject: Animal - DNA viruses
- Published Online:
A quantitative competitive PCR assay for human herpesvirus 6 (HHV-6) was developed. Firstly, viral burden was determined in the blood of 25 healthy persons. Using 1 µg of DNA, the prevalence of HHV-6 was 36% (9/25). Eight persons had viral loads of ⩽ 32 HHV-6 genomes/µg DNA. The viral burden in the ninth individual was 1·2 × 106 HHV-6 genome copies/µg DNA, which remained constant over a period of 10 months. This demonstrates the persistence of a high HHV-6 load in the absence of apparent disease. Secondly, HHV-6 burden was determined in 100 post-mortem tissues from seven AIDS patients and three controls. For all tissues combined, there was a statistically significant higher median viral load in AIDS patients (56 copies/µg DNA, range 0–43 321) compared to controls (10 copies/µg DNA, range 0–423) (P = 0·04). The precision and reproducibility of this assay will allow hypotheses concerning the pathogenic potential of HHV-6 to be tested quantitatively.
© Society for General Microbiology Ltd 1996 | Published by the Microbiology Society
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