Quantification of Human Herpesvirus 6 in Immunocompetent Persons and Post-mortem Tissues from AIDS Patients by PCR Clark, Duncan A. and Ait-Khaled, Mounir and Wheeler, Anne C. and Kidd, I. Michael and McLaughlin, James E. and Johnson, Margaret A. and Griffiths, Paul D. and Emery, Vincent C.,, 77, 2271-2275 (1996), doi = https://doi.org/10.1099/0022-1317-77-9-2271, publicationName = Microbiology Society, issn = 0022-1317, abstract= A quantitative competitive PCR assay for human herpesvirus 6 (HHV-6) was developed. Firstly, viral burden was determined in the blood of 25 healthy persons. Using 1 µg of DNA, the prevalence of HHV-6 was 36% (9/25). Eight persons had viral loads of ⩽ 32 HHV-6 genomes/µg DNA. The viral burden in the ninth individual was 1.2 × 106 HHV-6 genome copies/µg DNA, which remained constant over a period of 10 months. This demonstrates the persistence of a high HHV-6 load in the absence of apparent disease. Secondly, HHV-6 burden was determined in 100 post-mortem tissues from seven AIDS patients and three controls. For all tissues combined, there was a statistically significant higher median viral load in AIDS patients (56 copies/µg DNA, range 0–43 321) compared to controls (10 copies/µg DNA, range 0–423) (P = 0.04). The precision and reproducibility of this assay will allow hypotheses concerning the pathogenic potential of HHV-6 to be tested quantitatively., language=, type=