RT Journal Article SR Electronic(1) A1 Elster, Christine A1 Larsen, Kjeld A1 Gagnon, Jean A1 Ruigrok, Rob W. H. A1 Baudin, FlorenceYR 1997 T1 Influenza virus M1 protein binds to RNA through its nuclear localization signal JF Journal of General Virology, VO 78 IS 7 SP 1589 OP 1596 DO https://doi.org/10.1099/0022-1317-78-7-1589 PB Microbiology Society, SN 1465-2099, AB The RNA-binding activity of influenza A virus M1 protein was studied by cross-linking the protein to viral RNA followed by sequence analysis of the oligoribonucleotide bound to the protein as well as sequence analysis of the M1 peptide bound to the RNA. M1 was found to bind to RNA without any RNA sequence specificity, as verified in a series of filterbinding experiments using a large variety of nucleic acids including DNA. The peptide sequence that bound to the RNA was the RKLKR nuclear localization signal of M1. Site-specific mutagenesis of recombinant M1 showed that most of the basic residues in that region had to be mutated in order to inhibit RNA-binding. We also constructed an M1 mutant that no longer bound to RNA but which was still able to inhibit the in vitro transcription activity of isolated viral ribonucleoprotein, albeit to a lower extent. Mutation of the zinc-binding sequence had no effect on RNA-binding or transcription-inhibition activity., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-7-1589