1887

Abstract

Substitution of Val in Sendai virus (SeV) M protein generates non-functional polypeptides, characterized by their exclusion from virus particles and by their ability to interfere with virus particle production. These phenotypic traits correlate with a single-band PAGE migration profile, in contrast to wild-type M (M), which separates into two species, one of which is a phosphorylated form. The single-band migration is likely to result from a conformational change, as evidenced by the lack of maturation of a native epitope and by a particular tryptic digestion profile, and not from the phosphorylation of all M molecules, an assumption consistent with the PAGE migration feature. One of the M mutants (HA–M, an M protein carrying ThrMet and Val Glu substitutions tagged with an influenza virus haemagglutinin epitope) was characterized further in the context of SeV infection, i.e. under conditions of co-expression with M. HA–M is shown (i) to bind mainly to membrane fractions, (ii) not to co-precipitate M, as HA–M does, (iii) to interfere with the binding of nucleocapsids to membranes and (iv) to accumulate in perinuclear regions, in contrast to HA-M, which is also found at the cell periphery. Such mutants constitute potential tools for the identification of critical steps in paramyxovirus assembly and budding.

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1999-11-01
2024-04-19
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References

  1. Bächi T. 1980; Intramembrane structural differentiation in Sendai virus maturation. Virology 106:41–49
    [Google Scholar]
  2. Blumberg B. M., Rose K., Simona M. G., Roux L., Giorgi C., Kolakofsky D. 1984; Analysis of the Sendai virus M gene and protein. Journal of Virology 52:656–663
    [Google Scholar]
  3. Boyer J.-C., Haenni A.-L. 1994; Infectious transcripts and cDNA clones of RNA viruses. Virology 198:415–426
    [Google Scholar]
  4. Büechi M., Bächi T. 1982; Microscopy of internal structures of Sendai virus associated with the cytoplasmic surface of host membranes. Virology 120:349–359
    [Google Scholar]
  5. Calain P., Roux L. 1993; The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA. Journal of Virology 67:4822–4830
    [Google Scholar]
  6. Conzelmann K.-K. 1996; Genetic manipulation of non- segmented negative-strand RNA viruses. Journal of General Virology 77:381–389
    [Google Scholar]
  7. de Melo M., Mottet G., Örvell C., Roux L. 1992; Sendai virus M protein is found in two distinct isoforms defined by monoclonal antibodies. Virus Research 24:47–64
    [Google Scholar]
  8. Dubois-Dalcq M., Holmes K. V., Rentier B. 1984 Assembly of Enveloped RNA Viruses New York: Springer-Verlag;
  9. Egelman E. H., Wu S.-S., Amrein M., Portner A., Murti G. 1989; The Sendai virus nucleocapsid exists in at least four different helical states. Journal of Virology 63:2233–2243
    [Google Scholar]
  10. Engelhorn M., Stricker R., Roux L. 1993; Molecular cloning and characterization of a Sendai virus internal deletion defective RNA. Journal of General Virology 74:137–141
    [Google Scholar]
  11. Fekadu M., Chandler F. W., Harrison A. K. 1982; Pathogenesis of rabies in dogs inoculated with an Ethiopian rabies virus strain. Immunofluorescence, histologic and ultrastructural studies of the central nervous system. Archives of Virology 71:109–126
    [Google Scholar]
  12. Field J., Nikawa J.-I., Broek D., MacDonald B., Rodgers L., Wilson I. A., Lerner R. A., Wigler M. 1988; Purification of a RAS - responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Molecular and Cellular Biology 8:2159–2165
    [Google Scholar]
  13. Gaudin Y., Barge A., Ebel C., Ruigrok R. W. 1995; Aggregation of VSV M protein is reversible and mediated by nucleation sites: implications for viral assembly. Virology 206:28–37
    [Google Scholar]
  14. Giuffre R. M., Tovell D. R., Kay C. M., Tyrrell D. L. J. 1982; Evidence for an interaction between the membrane protein of a paramyxovirus and actin. Journal of Virology 42:963–968
    [Google Scholar]
  15. Heggeness M. H., Smith P. R., Choppin P. W. 1982; In vitro assembly of the nonglycosylated membrane protein (M) of Sendai virus. Proceedings of the National Academy of Sciences USA: 79:6232–6236
    [Google Scholar]
  16. Hewitt J. A. 1977; Studies on the subunit composition of the M-protein of Sendai virus. FEBS Letters 81:395–397
    [Google Scholar]
  17. Hewitt J. A., Nermut M. V. 1977; A morphological study of the M-protein of Sendai virus. Journal of General Virology 34:127–136
    [Google Scholar]
  18. Kingsbury D. W. 1974; The molecular biology of paramyxoviruses. Medical Microbiology and Immunology 160:73–83
    [Google Scholar]
  19. Lamb R. A., Choppin P. W. 1977; The synthesis of Sendai virus polypeptides in infected cells. III. Phosphorylation of polypeptides. Virology 81:382–397
    [Google Scholar]
  20. Lamb R. A., Kolakofsky D. 1996; Paramyxoviridae : the viruses and their replication. In Fields Virology pp 1177–1204 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  21. Markwell M. A., Fox C. F. 1980; Protein–protein interactions within paramyxoviruses identified by native disulfide bonding or reversible chemical cross-linking. Journal of Virology 33:152–166
    [Google Scholar]
  22. Mottet G., Portner A., Roux L. 1986; Drastic immunoreactivity changes between the immature and mature forms of the Sendai virus HN and F0 glycoproteins. Journal of Virology 59:132–141
    [Google Scholar]
  23. Mottet G., Curran J., Roux L. 1990; Intracellular stability of nonreplicating paramyxovirus nucleocapsids. Virology 176:1–7
    [Google Scholar]
  24. Mottet G., Mühlemann A., Tapparel C., Hoffmann F., Roux L. 1996; A Sendai virus vector leading to the efficient expression of mutant M proteins interfering with virus particle budding. Virology 221:159–171
    [Google Scholar]
  25. Nagai Y., Yoshida T., Yoshii S., Maeno K., Matsumoto T. 1975; Modification of normal cell surface by smooth membrane preparations from BHK-21 cells infected with Newcastle disease virus. Medical Microbiology and Immunology 161:175–188
    [Google Scholar]
  26. Nagai Y., Ogura H., Klenk H.-D. 1976; Studies on the assembly of the envelope of Newcastle disease virus. Virology 69:523–538
    [Google Scholar]
  27. Örvell C., Grandien M. 1982; The effects of monoclonal antibodies on biologic activities of structural proteins of Sendai virus. Journal of Immunology 129:2779–2787
    [Google Scholar]
  28. Peeples M. E. 1991; Paramyxovirus M proteins: pulling it all together and taking it on the road. In The Paramyxoviruses pp. 427–456 Edited by Kingsbury D. W. New York: Plenum Press;
    [Google Scholar]
  29. Ray R., Roux L., Compans R. W. 1991; Deletion mutants of paramyxoviruses. In The Paramyxoviruses pp. 457–479 Edited by Kingsbury D. W. New York: Plenum Press;
    [Google Scholar]
  30. Roux L., Holland J. J. 1979; Role of defective interfering particles of Sendai virus in persistent infections. Virology 93:91–103
    [Google Scholar]
  31. Sakaguchi T., Kiyotani K., Kato A., Asakawa M., Fujii Y., Nagai Y., Yoshida T. 1997; Phosphorylation of the Sendai virus M protein is not essential for virus replication either in vitro or in vivo. Virology 235:360–366
    [Google Scholar]
  32. Sanderson C. M., McQueen N. L., Nayak D. P. 1993; Sendai virus assembly: M protein binds to viral glycoproteins in transit through the secretory pathway. Journal of Virology 67:651–663
    [Google Scholar]
  33. Sanderson C. M., Wu H.-H., Nayak D. P. 1994; Sendai virus M protein binds independently to either the F or the HN glycoprotein in vivo. Journal of Virology 68:69–76
    [Google Scholar]
  34. Sanderson C. M., Avalos R., Kundu A., Nayak D. P. 1995; Interaction of Sendai viral F, HN, and M proteins with host cytoskeletal and lipid components in Sendai virus-infected BHK cells. Virology 209:701–707
    [Google Scholar]
  35. Stricker R., Mottet G., Roux L. 1994; The Sendai virus matrix protein appears to be recruited in the cytoplasm by the viral nucleocapsid to function in viral assembly and budding. Journal of General Virology 75:1031–1042
    [Google Scholar]
  36. Sugita K., Maru M., Sato K. 1974; A sensitive plaque assay for Sendai virus in an established line of monkey kidney cells. Japanese Journal of Microbiology 18:262–264
    [Google Scholar]
  37. Tashiro M., McQueen N. L., Seto J. T., Klenk H. D., Rott R. 1996; Involvement of the mutated M protein in altered budding polarity of a pantropic mutant, F1-R, of Sendai virus. Journal of Virology 70:5990–5997
    [Google Scholar]
  38. Tuchweber B., Desmoulière A., Bochaton-Piallat M.-L., Rubbia-Brandt L., Gabbiani G. 1996; Proliferation and phenotypic modulation of portal fibroblasts in the early stages of cholestatic fibrosis in the rat. Laboratory Investigation 74:265–278
    [Google Scholar]
  39. Tuffereau C., Roux L. 1988; Direct adverse effects of Sendai virus DI particles on virus budding and on M protein fate and stability. Virology 162:417–426
    [Google Scholar]
  40. Tyrrell D. L. J., Ehrnst A. 1979; Transmembrane communication in cells chronically infected with measles virus. Journal of Cell Biology 81:396–402
    [Google Scholar]
  41. Yoshida T., Nakayama Y., Nagura H., Toyoda T., Nishikawa K., Hamaguchi M., Nagai Y. 1986; Inhibition of the assembly of Newcastle disease virus by monensin. Virus Research 4:179–195
    [Google Scholar]
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