1887

Abstract

In the present study, the outcome of an inoculation of equine peripheral blood mononuclear cells (PBMC) with equine herpesvirus type 1 (EHV-1) was studied . Cytoplasmic and plasma membrane expression of viral antigens, intra- and extracellular virus titres, and plaque formation in co-culture were determined. EHV-1 replicated in monocytes, although in a highly restricted way. Viral antigens were found at maximum levels (8·7% of the monocytes) at 12 h post-infection. The infection was productive in 0·16% of the monocytes. The virus yield was 10 TCID per productive cell. In a population of resting lymphocytes, 0·9% of cells were infected and less than 0·05% produced infectious virus. After prestimulation with different mitogens, the number of infected lymphocytes increased four to twelve times. The susceptible lymphocytes were T-lymphocytes. In mitogen-stimulated lymphocytes, clear expression of viral antigens was found on the plasma membrane.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-1-21
2000-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/1/0810021a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-1-21&mimeType=html&fmt=ahah

References

  1. Albers, I., Kirchner, H. & Domke-Opitz, I. (1989). Resistance of human blood monocytes to infection with herpes simplex virus. Virology 169, 466-469.[CrossRef] [Google Scholar]
  2. Darzynkiewicz, Z., Williamson, B., Carswell, E. A. & Old, L. J. (1984). Cell cycle-specific effects of tumor necrosis factor. Cancer Research 44, 83-90. [Google Scholar]
  3. Doll, E. R. & Bryans, J. T. (1963). Immunisation of young horses against viral rhinopneumonitis. Cornell Veterinarian 53, 24-41. [Google Scholar]
  4. Dutta, S. K. & Myrup, A. C. (1983). Infectious center assay of intracellular virus and infective virus titer for equine mononuclear cells infected in vivo and in vitro with equine herpesvirus. Canadian Journal of Comparative Medicine 47, 64-69. [Google Scholar]
  5. Gibson, J. S., Slater, J. D., Awan, A. R. & Field, H. J. (1992). Pathogenesis of equine herpesvirus-1 in specific pathogen-free foals: primary and secondary infections and reactivation. Archives of Virology 123, 351-366.[CrossRef] [Google Scholar]
  6. McCulloch, J., Williamson, S. A., Powis, S. J. & Edington, N. (1993). The effect of EHV1 infection upon circulating leucocyte populations in the natural equine host. Veterinary Microbiology 37, 147-161.[CrossRef] [Google Scholar]
  7. Mettenleiter, T. C. (1989). Glycoprotein gIII deletion mutants of pseudorabies virus are impaired in virus entry. Virology 171, 623-625.[CrossRef] [Google Scholar]
  8. Mintz, L., Drew, W. L., Hoo, R. & Finley, T. N. (1980). Age-dependent resistance of human alveolar macrophages to herpes simplex virus. Infection and Immunity 28, 417-420. [Google Scholar]
  9. Mumford, J. A., Rossdale, P. D., Jesset, D. M., Gann, S., Ousey, J. & Cook, R. F. (1987). Serological and virological investigations of an equid herpesvirus 1 (EHV1) abortion storm on a stud farm in 1985. Journal of Reproduction and Fertility Supplement 35, 509-518. [Google Scholar]
  10. Nauwynck, H. J. & Pensaert, M. B. (1994). Virus production and viral antigen expression in porcine blood monocytes inoculated with pseudorabies virus. Archives of Virology 137, 69-79.[CrossRef] [Google Scholar]
  11. Nyaga, P. N. & McKercher, D. G. (1980). Pathogenesis of bovine herpesvirus-1 (BHV1) infections: interactions of the virus with peripheral bovine blood cellular components. Comparative Immunobiology, Microbiology and Infectious Diseases 2, 587-602. [Google Scholar]
  12. Plaeger-Marshall, S. & Smith, J. W. (1978). Inhibition of mitogen- and antigen-induced lymphocyte blastogenesis by herpes simplex virus. Journal of Infectious Diseases 138, 506-511.[CrossRef] [Google Scholar]
  13. Rouse, B. T. & Babiuk, L. A. (1975). Host defence mechanisms against infectious bovine rhinotracheitis virus II. Inhibition of viral plaque formation by immune peripheral blood lymphocytes. Cellular Immunology 17, 43-56.[CrossRef] [Google Scholar]
  14. Schang, L. M., Phillips, J. & Schaffer, P. A. (1998). Requirement for cellular cyclin-dependent kinases in herpes simplex virus replication and transcription. Journal of Virology 72, 5626-5637. [Google Scholar]
  15. Scott, J. C., Dutta, S. K. & Myrup, A. C. (1983). In vivo harbouring of equine herpesvirus-1 in leukocyte populations and subpopulations and their quantitation from experimentally infected ponies. American Journal of Veterinary Research 44, 1344-1348. [Google Scholar]
  16. Terada, N., Lucas, J. J. & Gelfand, E. W. (1991). Differential regulation of the tumor suppressor molecules, retinoblastoma susceptibility gene product (Rb) and p53, during cell cycle progression of normal human T-cells. Journal of Immunology 147, 698-704. [Google Scholar]
  17. Teute, H., Braun, R., Kirchner, H., Becker, H. & Munk, K. (1983). Replication of herpes simplex virus in human T lymphocytes. Intervirology 20, 32-41.[CrossRef] [Google Scholar]
  18. Tumas, D. B., Brassfield, A. L., Travenor, A. S., Hines, M. T., Davis, W. C. & McGuire, T. C. (1994). Monoclonal antibodies to the equine CD2 T lymphocyte marker, to a pan-granulocyte/monocyte marker and to a unique pan-B lymphocyte marker. Immunobiology 192, 48-64.[CrossRef] [Google Scholar]
  19. Wang, F., Pang, V. F. & Hahn, E. C. (1988). Flow cytometric analysis of porcine peripheral blood leukocytes infected with Aujeszky’s disease virus. Journal of Leukocyte Biology 43, 256-264. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-1-21
Loading
/content/journal/jgv/10.1099/0022-1317-81-1-21
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error