Molecular analysis of a serotype 8 human astrovirus genome
Méndez-Toss, Martha and Romero-Guido, Pedro and Munguía, Maria Elena and Méndez, Ernesto and Arias, Carlos F.,, 81, 2891-2897 (2000),
doi = https://doi.org/10.1099/0022-1317-81-12-2891,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Human astroviruses are an important cause of gastroenteritis. As part of a molecular epidemiological study carried out in Mexico a human astrovirus isolate, Yuc-8, was adapted to grow in CaCo-2 cells, and its entire genome was sequenced. A 15 amino acid deletion in ORF1a, which has been associated with adaptation of astroviruses to grow in cells other than CaCo-2, was present in Yuc-8. Comparative sequence analysis of the Yuc-8 ORF2 with reported human astrovirus sequences revealed that this isolate belongs to genotype (serotype) 8. Two distinct domains in ORF2 were observed: an amino-terminal domain (residues 1 to 415), with identities higher than 81% among the strains analysed, and a carboxy-terminal domain (residues 416 to 782) with identities between 36 and 60%. Two non-superimposable phylogenetic trees were generated by separate analysis of these two domains, suggesting that a differential selective pressure is exerted along the structural polyprotein.,
language=,
type=