1887

Abstract

It was recently shown that the class II membrane glycoprotein G of bovine respiratory syncytial virus (BRSV) is integrated into the envelope of recombinant bovine herpesvirus-1 (BHV-1) virions in the correct orientation. To verify the hypothesis that the membrane anchor of BRSV G might be suitable to target heterologous polypeptides into the membrane of recombinant BHV-1 particles, an open reading frame encoding a fusion protein between amino acids 1 to 71 of the BRSV G glycoprotein and the green fluorescent protein (TMIIGFP) was recombined into the genome of BHV-1. The resulting recombinant BHV-1/eTMIIGFP had growth properties similar to those of wild-type BHV-1. Live-cell analysis of cells infected with BHV-1/eTMIIGFP indicated that the fusion protein localized to the cell surface. Immunoprecipitations and virus neutralization assays using a GFP-specific antiserum proved that TMIIGFP was incorporated as a class II membrane protein into virions.

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2000-04-01
2024-04-19
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References

  1. Brideau, A. D., Banfield, B. W. & Enquist, L. W. (1998). The US9 gene product of pseudorabies virus, an alphaherpesvirus, is a phosphorylated, tail-anchored type II membrane protein.Journal of Virology 72, 4560-4570. [Google Scholar]
  2. Dolter, K. E., King, S. R. & Holland, T. C. (1993). Incorporation of CD4 into virions by a recombinant herpes simplex virus.Journal of Virology 67, 189-195. [Google Scholar]
  3. Fehler, F., Herrmann, J. M., Saalmüller, A., Mettenleiter, T. C. & Keil, G. M. (1992). Glycoprotein IV of bovine herpesvirus 1-expressing cell line complements and rescues a conditionally lethal virus mutant.Journal of Virology 66, 831-839. [Google Scholar]
  4. Furze, J., Wertz, G., Lerch, R. & Taylor, G. (1994). Antigenic heterogeneity of the attachment protein of bovine respiratory syncytial virus.Journal of General Virology 75, 363-370.[CrossRef] [Google Scholar]
  5. Furze, J. M., Roberts, S. R., Wertz, W. G. & Taylor, G. (1997). Antigenically distinct G glycoproteins of BRSV strains share a high degree of genetic homogeneity.Virology 231, 48-58.[CrossRef] [Google Scholar]
  6. Gibbs, E. P. J. & Rweyemamu, M. M. (1977). Bovine herpesviruses. Part 1. Bovine herpesvirus 1.Veterinary Bulletin 47, 317-343. [Google Scholar]
  7. Kühnle, G., Collins, R. A., Scott, J. E. & Keil, G. M. (1996). Bovine interleukins 2 and 4 expressed in recombinant bovine herpesvirus 1 are biologically active secreted glycoproteins.Journal of General Virology 77, 2231-2240.[CrossRef] [Google Scholar]
  8. Kühnle, G., Heinze, A., Schmitt, J., Giesow, K., Taylor, G., Morrison, I., Rijsewijk, F. A. M., van Oirschot, J. T. & Keil, G. M. (1998). The class II membrane glycoprotein G of bovine respiratory syncytial virus, expressed from a synthetic open reading frame, is incorporated into virions of recombinant bovine herpesvirus 1.Journal of Virology 72, 3804-3811. [Google Scholar]
  9. Kurilla, M. G., Heinemann, T., Davenport, L. C., Kieff, E. & Hutt-Fletcher, L. M. (1995). A novel Epstein–Barr virus glycoprotein gp150 expressed from the BDLF3 open reading frame.Virology 209, 108-121.[CrossRef] [Google Scholar]
  10. Lerch, R. A., Anderson, K. & Wertz, G. W. (1990). Nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment glycoprotein G of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus.Journal of Virology 64, 5559-5567. [Google Scholar]
  11. Meyer, G., Hanon, E., Georlette, D., Pastoret, P.-P. & Thiry, E. (1998). Bovine herpesvirus type 1 glycoprotein H is essential for penetration and propagation in cell culture.Journal of General Virology 79, 1983-1987. [Google Scholar]
  12. Miethke, A., Keil, G. M., Weiland, F. & Mettenleiter, T. C. (1995). Unidirectional complementation between glycoprotein B homologues of pseudorabies virus and bovine herpesvirus 1 is determined by the carboxy-terminal part of the molecule.Journal of General Virology 76, 1623-1635.[CrossRef] [Google Scholar]
  13. Otsuka, H. & Xuan, X. (1996). Construction of bovine herpesvirus-1 (BHV-1) recombinants which express pseudorabies virus (PRV) glycoproteins gB, gC, gD and gE.Archives of Virology 141, 57-71.[CrossRef] [Google Scholar]
  14. Roizman, B., Desrosier, R. C., Fleckenstein, B., Lopes, C., Minson, A. C. & Studdert, M. J. (1992). The family herpesviridae: an update.Archives of Virology 123, 425-449.[CrossRef] [Google Scholar]
  15. Schmitt, J., Becher, P., Thiel, H.-J. & Keil, G. M. (1999). Expression of bovine viral diarrhoea virus glycoprotein E2 by bovine herpesvirus-1 from a synthetic ORF and incorporation of E2 into recombinant virions.Journal of General Virology 80, 2839-2848. [Google Scholar]
  16. Schröder, C. & Keil, G. M. (1999). Bovine herpesvirus 1 requires glycoprotein H for infectivity and direct spreading and glycoproteins gHW450 and gB for glycoprotein D-independent cell-to-cell spread.Journal of General Virology 80, 57-61. [Google Scholar]
  17. Schwyzer, M. & Ackermann, M. (1996). Molecular virology of ruminant herpesviruses.Veterinary Microbiology 53, 17-29.[CrossRef] [Google Scholar]
  18. van Drunen Littel-van den Hurk, S. & Babiuk, L. A. (1986). Synthesis and processing of bovine herpesvirus 1 glycoproteins.Journal of Virology 59, 401-410. [Google Scholar]
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