1887

Abstract

The entire nucleotide sequences of all six internal genes of six human H5N1 influenza A viruses isolated in Hong Kong in 1997 were analysed in detail from a phylogenetic point of view and compared with the evolutionary patterns of the haemagglutinin and neuraminidase genes. Despite being isolated within a single year in the same geographical location, human H5N1 viruses were characterized by a variety of amino acid substitutions in the ribonucleoprotein complex [PB2, PB1, PA and nucleoprotein (NP)] as well as the matrix (M) proteins 1 and 2 and nonstructural (NS) proteins 1 and 2. The presence of previously reported amino acid sequences specific for human strains was confirmed in the PB2, PA, NP and M2 proteins. Nucleotide and amino acid sequence identities of the six internal genes of H5N1 viruses examined here were separated into at least two variant groups. In agreement with the above result, phylogenetic trees of the six internal genes of human H5N1 viruses were generally composed of two minor clades. Additionally, variable dendrogram topologies suggested that reassortment among viruses contributed further to the genetic variability of these viruses. As a result, it became clear that human H5N1 viruses are characterized by divergent gene constellations, suggesting the possible occurrence of genetic reassortment between viruses of the two evolutionary lineages.

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2000-05-01
2024-03-28
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References

  1. Banks, J., Speidel, E. & Alexander, D. J. (1998). Characterisation of an avian influenza A virus isolated from a human – is an intermediate host necessary for the emergence of pandemic influenza viruses?Archives of Virology 143, 781-787.[CrossRef] [Google Scholar]
  2. Bender, C., Hall, H., Huang, J., Klimov, A., Cox, N., Hay, A., Gregory, V., Cameron, K., Lim, W. & Subbarao, K. (1999). Characterization of the surface proteins of influenza A (H5N1) viruses isolated from humans in 1997–1998.Virology 254, 115-123.[CrossRef] [Google Scholar]
  3. Buckler-White, A. J. & Murphy, B. R. (1986). Nucleotide sequence analysis of the nucleoprotein gene of an avian and a human influenza virus strain identifies two classes of nucleoproteins.Virology 155, 345-355.[CrossRef] [Google Scholar]
  4. Chomczynski, P. & Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction.Analytical Biochemistry 162, 156-159. [Google Scholar]
  5. Claas, E. C., Osterhaus, A. D., van Beek, R., De Jong, J. C., Rimmelzwaan, G. F., Senne, D. A., Krauss, S., Shortridge, K. F. & Webster, R. G. (1998). Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus. Lancet 351, 472–477; erratum 1292.[CrossRef] [Google Scholar]
  6. Crosby, A. W. (1976). Flu and the American expeditionary force. In Epidemic and Peace 1918, pp. 145–170. Westport, CT: Green Wood Press.
  7. de Jong, J. C., Claas, E. C., Osterhaus, A. D., Webster, R. G. & Lim, W. L. (1997). A pandemic warning?Nature 389, 554. [Google Scholar]
  8. Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap.Evolution 39, 783-791.[CrossRef] [Google Scholar]
  9. Felsenstein, J. (1995). PHYLIP (Phylogeny Inference Package) version 3.57c. Distributed by the author. Department of Genetics, University of Washington, Seattle, WA, USA.
  10. Gao, P., Watanabe, S., Ito, T., Goto, H., Wells, K., McGregor, M., Cooley, A. J. & Kawaoka, Y. (1999). Biological heterogeneity, including systemic replication in mice, of H5N1 influenza A virus isolates from humans in Hong Kong.Journal of Virology 73, 3184-3189. [Google Scholar]
  11. Horimoto, T., Rivera, E., Pearson, J., Senne, D., Krauss, S., Kawaoka, Y. & Webster, R. G. (1995). Origin and molecular changes associated with emergence of a highly pathogenic H5N2 influenza virus in Mexico.Virology 213, 223-230.[CrossRef] [Google Scholar]
  12. Ito, T., Gorman, O. T., Kawaoka, Y., Bean, W. J. & Webster, R. G. (1991). Evolutionary analysis of the influenza A virus M gene with comparison of the M1 and M2 proteins.Journal of Virology 65, 5491-5498. [Google Scholar]
  13. Kawaoka, Y., Nestorowicz, A., Alexander, D. J. & Webster, R. G. (1987). Molecular analyses of the hemagglutinin genes of H5 influenza viruses: origin of a virulent turkey strain. Virology 158, 218–227; erratum 159, 196.[CrossRef] [Google Scholar]
  14. Kawaoka, Y., Krauss, S. & Webster, R. G. (1989). Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics.Journal of Virology 63, 4603-4608. [Google Scholar]
  15. Lindstrom, S. E., Hiromoto, Y., Nerome, R., Omoe, K., Sugita, S., Yamazaki, Y., Takahashi, T. & Nerome, K. (1998). Phylogenetic analysis of the entire genome of influenza A (H3N2) viruses from Japan: evidence for genetic reassortment of the six internal genes.Journal of Virology 72, 8021-8031. [Google Scholar]
  16. Lu, X., Tumpey, T. M., Morken, T., Zaki, S. R., Cox, N. J. & Katz, J. M. (1999). A mouse model for the evaluation of pathogenesis and immunity to influenza A (H5N1) viruses isolated from humans.Journal of Virology 73, 5903-5911. [Google Scholar]
  17. Murphy, B. R. & Webster, R. G. (1996). Orthomyxoviruses. In Fields Virology, pp. 1397-1445. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  18. Murphy, B. R., Buckler-White, A. J., London, W. T. & Snyder, M. H. (1989). Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys.Vaccine 7, 557-561.[CrossRef] [Google Scholar]
  19. Nei, M. & Gojobori, T. (1986). Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions.Molecular Biology and Evolution 3, 418-426. [Google Scholar]
  20. Nerome, K., Sakamoto, S., Yano, N., Yamamoto, T., Kobayashi, S., Webster, R. G. & Oya, A. (1983). Antigenic characteristics and genome composition of a naturally occurring recombinant influenza virus isolated from a pig in Japan.Journal of General Virology 64, 2611-2620.[CrossRef] [Google Scholar]
  21. Nerome, K., Yoshioka, Y., Sakamoto, S., Yasuhara, H. & Oya, A. (1985). Characterization of a 1980-swine recombinant influenza virus possessing H1 hemagglutinin and N2 neuraminidase similar to that of the earliest Hong Kong (H3N2) virus.Archives of Virology 86, 197-211.[CrossRef] [Google Scholar]
  22. Nerome, K., Kanegae, Y., Shortridge, K. F., Sugita, S. & Ishida, M. (1995). Genetic analysis of porcine H3N2 viruses originating in southern China.Journal of General Virology 76, 613-624.[CrossRef] [Google Scholar]
  23. Ouchi, A., Nerome, K., Kanegae, Y., Ishida, M., Nerome, R., Hayashi, K., Hashimoto, T., Kaji, M., Kaji, Y. & Inaba, Y. (1996). Large outbreak of swine influenza in southern Japan caused by reassortant (H1N2) influenza viruses: its epizootic background and characterization of the causative viruses.Journal of General Virology 77, 1751-1759.[CrossRef] [Google Scholar]
  24. Saitou, N. & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees.Molecular Biology and Evolution 4, 406-425. [Google Scholar]
  25. Scholtissek, C., Rohde, W., Von Hoyningen, V. & Rott, R. (1978). On the origin of the human influenza virus subtypes H2N2 and H3N2.Virology 87, 13-20.[CrossRef] [Google Scholar]
  26. Scholtissek, C., Burger, H., Kistner, O. & Shortridge, K. F. (1985). The nucleoprotein as a possible major factor in determining host specificity of influenza H3N2 viruses.Virology 147, 287-294.[CrossRef] [Google Scholar]
  27. Shortridge, K. F., Zhou, N. N., Guan, Y., Gao, P., Ito, T., Kawaoka, Y., Kodihalli, S., Krauss, S., Markwell, D., Murti, K. G., Norwood, M., Senne, D., Sims, L., Takada, A. & Webster, R. G. (1998). Characterization of avian H5N1 influenza viruses from poultry in Hong Kong.Virology 252, 331-342.[CrossRef] [Google Scholar]
  28. Shu, L. L., Lin, Y. P., Wright, S. M., Shortridge, K. F. & Webster, R. G. (1994). Evidence for interspecies transmission and reassortment of influenza A viruses in pigs in southern China.Virology 202, 825-833.[CrossRef] [Google Scholar]
  29. Suarez, D. L., Perdue, M. L., Cox, N., Rowe, T., Bender, C., Huang, J. & Swayne, D. E. (1998). Comparisons of highly virulent H5N1 influenza A viruses isolated from humans and chickens from Hong Kong.Journal of Virology 72, 6678-6688. [Google Scholar]
  30. Subbarao, E. K., London, W. & Murphy, B. R. (1993). A single amino acid in the PB2 gene of influenza A virus is a determinant of host range.Journal of Virology 67, 1761-1764. [Google Scholar]
  31. Subbarao, K., Klimov, A., Katz, J., Regnery, H., Lim, W., Hall, H., Perdue, M., Swayne, D., Bender, C., Huang, J., Hemphill, M., Rowe, T., Shaw, M., Xu, X., Fukuda, K. & Cox, N. (1998). Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness.Science 279, 393-396.[CrossRef] [Google Scholar]
  32. Yuen, K. Y., Chan, P. K., Peiris, M., Tsang, D. N., Que, T. L., Shortridge, K. F., Cheung, P. T., To, W. K., Ho, E. T., Sung, R. & Cheng, A. F. (1998). Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus.Lancet 351, 467-471.[CrossRef] [Google Scholar]
  33. Zhou, N. N., Shortridge, K. F., Claas, E. C. J., Krauss, S. L. & Webster, R. G. (1999). Rapid evolution of H5N1 influenza viruses in chickens in Hong Kong.Journal of Virology 73, 3366-3374. [Google Scholar]
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