1887

Abstract

Two serotypes have been identified in infectious bursal disease virus (IBDV), a member of the family . A reverse genetics system was used for generation of chimeras in genome segment A of the two serotypes, in which the complete viral VP5 gene and 3′ noncoding region (NCR), or parts thereof, were exchanged. The engineered viruses were characterized and in comparison to serotype I and II IBDV. Our results show that IBDV chimeras exhibit a different phenotype in cell culture compared to the wild-type viruses. In -cultivated bursal-derived cells, chimeric viruses infected B lymphocytes, as does serotype I IBDV. Surprisingly, serotype II virus was also able to infect -cultivated bursal cells, but these were neither B lymphocytes nor macrophages. After infection of susceptible chickens all chimeras replicated in the bursa of Fabricius (BF), and three chimeric viruses caused mild depletion of bursal cells. In contrast, after infection of chickens with a chimeric IBDV containing exchanged VP5 as well as 3′-NCR, no depletion was detectable. The serotype II strain did not replicate in the BF nor did it cause depletion of bursal cells. Thus, the origin of VP5 does not explain the different pathotype of IBDV serotype I and II.

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2001-01-01
2024-04-25
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References

  1. Bayliss C. D., Spies U., Shaw K., Peters R. W., Papageorgiou A., Müller H., Boursnell M. E. G. 1990; A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. Journal of General Virology 71:1303–1312
    [Google Scholar]
  2. Birghan C., Mundt E., Gorbalenya A. 2000; A non-canonical Lon proteinase deficient of the ATPase domain employs the Ser–Lys catalytic dyad to impose broad control over the life cycle of a double-stranded RNA virus. EMBO Journal 19:114–123
    [Google Scholar]
  3. Cosgrove A. S. 1962; An apparently new disease of chickens – avian nephrosis. Avian Diseases 6:385–389
    [Google Scholar]
  4. Hudson P. J., McKern N. M., Power B. E., Azad A. A. 1986; Genomic structure of the large RNA segment of infectious bursal disease virus. Nucleic Acids Research 14:5001–5012
    [Google Scholar]
  5. Hirai K., Calnek B. W. 1979; In vitro replication of infectious bursal disease virus in established lymphoid cell lines and chicken B lymphocytes. Infection and Immunity 25:964–970
    [Google Scholar]
  6. Ismail N. M., Saif Y. M., Moorhead P. D. 1988; Lack of pathogenicity of five serotype 2 infectious bursal disease viruses in chickens. Avian Diseases 32:757–759
    [Google Scholar]
  7. Jackwood D. J., Saif Y. M., Hughes J. H. 1982; Characteristics and serologic studies of two serotypes of infectious bursal disease virus in turkey. Avian Diseases 26:871–882
    [Google Scholar]
  8. Jeurissen S. H. M., Janse E. M., Koch G., De Boe G. F. 1988; The monoclonal antibody CVI-ChNL-68.1 recognizes cells of the monocyte–macrophage lineage in chickens. Developmental and Comparative Immunology 12:855–864
    [Google Scholar]
  9. Lui X., Giambrone J. J., Hoerr F. J. 2000; In situ hybridization, immunohistochemistry, and in situ reverse transcription-polymerase chain reaction for detection of infectious bursal disease virus. Avian Diseases 44:161–169
    [Google Scholar]
  10. McFerran J. B., McNulty M. S., Killop E. R., Connor T. J., McCracken R. M., Collins P. S., Allan G. M. 1980; Isolation and serological studies with infectious bursal disease virus from fowl, turkeys and ducks: demonstration of a second serotype. Avian Pathology 9:395–404
    [Google Scholar]
  11. Mundt E. 1999; Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2. Journal of General Virology 80:2067–2076
    [Google Scholar]
  12. Mundt E., Müller H. 1995; Complete nucleotide sequences of 5′- and 3′-noncoding regions of both genome segments of different strains of infectious bursal disease virus. Virology 209:209–218
    [Google Scholar]
  13. Mundt E., Vakharia V. N. 1996; Synthetic transcripts of double-stranded birnavirus genome are infectious. Proceedings of the National Academy of Sciences, USA 93:11131–11136
    [Google Scholar]
  14. Mundt E., Beyer J., Müller H. 1995; Identification of a novel viral protein in infectious bursal disease virus-infected cells. Journal of General Virology 76:437–443
    [Google Scholar]
  15. Mundt E., Köllner B., Kretzschmar D. 1997; VP5 of infectious bursal disease virus (IBDV) is not essential for viral replication in cell culture. Journal of Virology 71:5647–5651
    [Google Scholar]
  16. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. (editors) 1995; Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses. Vienna & New York: Springer-Verlag;
    [Google Scholar]
  17. Nieper H., Müller H. 1996; Susceptibility of chicken lymphoid cells to infectious bursal disease virus does not correlate with the presence of specific binding sites. Journal of General Virology 77:1229–1237
    [Google Scholar]
  18. Nieper H., Teifke J. P., Jungmann A., Löhr C. V., Müller H. 1999; Infected and apoptotic cells in the IBDV-infected bursa of Fabricius, studied by double-labelling techniques. Avian Pathology 28:279–285
    [Google Scholar]
  19. Öppling V., Müller H., Becht H. 1991; The structural polypeptide VP3 of infectious bursal disease virus carries group- and serotype-specific epitopes. Journal of General Virology 72:2275–2278
    [Google Scholar]
  20. Rothwell C. J., Vervelde L., Davison T. F. 1996; Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Veterinary Immunology and Immunopathology 55:225–234
    [Google Scholar]
  21. Schröder A., van Loon A. A. W. M., Goovaerts D., Mundt E. 2000; Chimeras in noncoding regions between serotype I and II of segment A of infectious bursal disease virus are viable and show pathogenic phenotype in chickens. Journal of General Virology 81:533–540
    [Google Scholar]
  22. Spies U., Müller H., Becht H. 1987; Properties of RNA polymerase activity associated with infectious bursal disease virus and characterization of its reaction products. Virus Research 8:127–140
    [Google Scholar]
  23. Spies U., Müller H., Becht H. 1989; Nucleotide sequence of infectious bursal disease virus segment A delineates two major open reading frames. Nucleic Acids Research 17:7982
    [Google Scholar]
  24. Teifke J. P., Löhr C. V., Shirasawa H. 1998; Detection of canine oral papillomavirus (COPV)-DNA in canine oral squamous cell carcinomas and p53 overexpressing skin papillomas of the dog using the polymerase chain reaction and nonradioactive in situ hybridization. Veterinary Microbiology 60:119–130
    [Google Scholar]
  25. Teifke J. P., Löhr C. V., Marschang R. E., Osterrieder N., Posthaus H. 2000; Detection of chelonid herpesvirus DNA by nonradioactive in situ hybridization in tissues from tortoises suffering from stomatitis–rhinitis-complex in Europe and North America. Veterinary Pathology 37: (in press
    [Google Scholar]
  26. Yao K., Goodwin M. A., Vakharia V. N. 1998; Generation of a mutant infectious bursal disease virus that does not cause bursal lesions. Journal of Virology 72:2647–2657
    [Google Scholar]
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