@article{mbs:/content/journal/jgv/10.1099/0022-1317-82-4-735, author = "Johansson, Magnus and Brooks, Andrew J. and Jans, David A. and Vasudevan, Subhash G.", title = "A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-β and the viral helicase, NS3", journal= "Journal of General Virology", year = "2001", volume = "82", number = "4", pages = "735-745", doi = "https://doi.org/10.1099/0022-1317-82-4-735", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-4-735", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The dengue virus RNA-dependent RNA polymerase, NS5, and the protease/helicase, NS3, are multidomain proteins that have been shown to interact both in vivo and in vitro. A hyperphosphorylated form of NS5 that does not interact with NS3 has been detected in the nuclei of virus-infected cells, presumably as the result of the action of a functional nuclear localization sequence within the interdomain region of NS5 (residues 369–405). In this study, it is shown by using the yeast two-hybrid system that the C-terminal region of NS3 (residues 303–618) interacts with the N-terminal region of NS5 (residues 320–368). Further, it is shown that this same region of NS5 is also recognized by the cellular nuclear import receptor importin-β. The interaction between NS5 and importin-β and competition by NS3 with the latter for the same binding site on NS5 were confirmed by pull-down assays. The direct interaction of importin-β with NS5 has implications for the mechanism by which this normally cytoplasmic protein may be targetted to the nucleus.", }