1887

Abstract

Using a panel of new monoclonal antibodies (mAbs), five neutralizing, conformation-dependent sites have been identified on the antigenic variant of swine vesicular disease virus (SVDV) circulating currently. In studies on the antigenic conservation of these sites, the four antigenic/genetic groups of SVDV described showed distinguishable patterns, confirming this classification. By sequencing mAb-resistant mutants, the five sites have been mapped precisely and localized on a three-dimensional model of the SVDV capsid. All were found to be orientated, to a different extent, towards the external surface of the capsid. Three of the five sites, located in VP1, VP2 and VP3, correspond to epitopes identified previously in historic isolates as sites 1, 2a and 3b, respectively. Another site, site IV, which maps to position 258 of VP1, corresponds to an epitope reported recently and is described in this study to be specific for isolates of the most recent antigenic group of SVDV. A fifth site is described for the first time and corresponds to the unique neutralizing site that is common to both SVDV and coxsackie B5 virus; it maps to positions 95 and 98 of VP1, but may also include positions nearby that belong to site 1 on the BC-loop of VP1, suggesting the classification of site Ia. These results may have useful diagnostic and epidemiological applications, since mAbs to the new conserved site Ia provide universal reagents for SVDV detection systems, while the specificity of mAbs to site IV make them unique markers for the most recent strains of SVDV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-1-35
2002-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/1/0830035a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-1-35&mimeType=html&fmt=ahah

References

  1. Borrego B., Bugnetti M., Berlinzani A., De Simone F., Brocchi E. 2000a; Stability of the antigenic profile of swine vesicular disease virus isolates collected during 8 years (1992–1999). In Veterinary Virology in the New Millennium , pp. 169–170. Proceedings of the 5th International Congress of the European Society for Veterinary Virology Brescia, Italy: 27–30 August 2000
    [Google Scholar]
  2. Borrego B., García-Ranea J. A., Douglas A., Jiménez-Clavero M. A., Brocchi E. 2000b; Mapping of linear epitopes on the capsid proteins of swine vesicular disease virus using monoclonal antibodies. In Veterinary Virology in the New Millennium , pp. 165–166. Proceedings of the 5th International Congress of the European Society for Veterinary Virology, (Brescia, Italy. 27–30 August 2000)
    [Google Scholar]
  3. Brocchi E., Gamba D., Poumarat F., Martel J. L., De Simone F. 1993; Improvements in the diagnosis of contagious bovine pleuropneumonia through the use of monoclonal antibodies. Revue Scientifique et Technique (Office International Des Epizooties) 12:559–570
    [Google Scholar]
  4. Brocchi E., Berlinzani A., Gamba D., De Simone F. 1995; Development of two novel monoclonal antibody-based ELISAs for the detection of antibodies and the identification of swine isotypes against swine vesicular disease virus. Journal of Virological Methods 52:155–167
    [Google Scholar]
  5. Brocchi E., Zhang G., Knowles N. J., Wilsden G., McCauley J. W., Marquardt O., Ohlinger V. F., De Simone F. 1997; Molecular epidemiology of recent outbreaks of swine vesicular disease: two genetically and antigenically distinct variants in Europe, 1987–94. Epidemiology and Infection 118:51–61
    [Google Scholar]
  6. Brown F., Hyslop N. G., Crick J., Morrow A. W. 1963; The use of acetylethylenimine in the production of inactivated foot-and-mouth-disease vaccines. Journal of Hygiene 61:337–344
    [Google Scholar]
  7. Brown F., Talbot P., Burrows R. 1973; Antigenic differences between isolates of swine vesicular disease virus and their relationship to coxsackie B5 virus. Nature 245:315–316
    [Google Scholar]
  8. Chenard G., Bloemraad M., Kramps J. A., Terpstra C., Dekker A. 1998; Validation of a monoclonal antibody-based ELISA to detect antibodies directed against swine vesicular disease virus. Journal of Virological Methods 75:105–112
    [Google Scholar]
  9. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thyocyanate–phenol–chloroform extraction. Analytical Biochemistry. 162156–159
  10. Fallacara F., Pacciarini M., Bugnetti M., Berlinzani A., Brocchi E. 2000; Detection of swine vesicular disease virus in faeces samples by immune-PCR assay. In Veterinary Virology in the New Millennium pp 173–174 Proceedings of the 5th International Congress of the European Society for Veterinary Virology, (Brescia, Italy. 27–30 August 2000)
    [Google Scholar]
  11. Galfrè G., Milstein C. 1981; Preparation of monoclonal antibodies: strategies and procedures. Methods in Enzymology 73:3–46
    [Google Scholar]
  12. Graves J. H. 1973; Serological relationship of swine vesicular disease and coxsackie B5 virus. Nature 245:314–315
    [Google Scholar]
  13. Harlow E., Lane D. 1988; Immunoblotting protocols. In Antibodies: a Laboratory Manual pp 479–510 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Inoue T., Suzuki T., Sekiguchi K. 1989; The complete nucleotide sequence of swine vesicular disease virus. Journal of General Virology 70:919–934
    [Google Scholar]
  15. Jiménez-Clavero M. A., Escribano-Romero E., Sanchez-Vizcaino J. M., Ley V. 1998; Molecular cloning, expression and immunological analysis of the capsid precursor polypeptide (P1) from swine vesicular disease virus. Virus Research 57:163–170
    [Google Scholar]
  16. Jiménez-Clavero M. A., Douglas A. J., Lavery P., García-Ranea J. A., Ley V. 2000; Immune recognition of swine vesicular disease virus structural proteins: novel antigenic regions that are not exposed in the capsid. Virology 270:76–83
    [Google Scholar]
  17. Jiménez-Clavero M. A., Escribano-Romero E., Douglas A. J., Ley V. 2001; The N-terminal region of the VP1 protein of swine vesicular disease virus contains a neutralization site that arises upon cell attachment and is involved in viral entry. Journal of Virology 75:1044–1047
    [Google Scholar]
  18. Kabsch W., Sander C. 1983; Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers 22:2577–2637
    [Google Scholar]
  19. Kanno T., Inoue T., Wang Y., Sarai A., Yamaguchi S. 1995; Identification of the location of antigenic sites of swine vesicular disease virus with neutralization-resistant mutants. Journal of General Virology 76:3099–3106
    [Google Scholar]
  20. King A. M. Q., Brown F., Christian P., Hovi T., Hyypiä T., Knowles N. J., Lemon S. M., Minor P. D., Palmenberg A. C., Skern T., Stanway G. 2000; Picornaviridae . In Virus Taxonomy. Seventh Report of the International Committee for the Taxonomy of Viruses. pp 657–673 Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B. New York & San Diego: Academic Press;
  21. Lin F., Mackay D. K., Knowles N. J. 1998; The persistence of swine vesicular disease virus infection in pigs. Epidemiology and Infection 121:459–472
    [Google Scholar]
  22. Mateu M. G. 1995; Antibody recognition of picornaviruses and escape from neutralization: a structural view. Virus Research 38:1–24
    [Google Scholar]
  23. Nardelli L., Lodetti G., Gualandi G. L., Burrows R., Goodridge D., Brown F., Cartwright B. 1968; A foot-and-mouth disease syndrome in pigs caused by an enterovirus. Nature 219:1275–1276
    [Google Scholar]
  24. Nijhar S., Mackay D. K., Brocchi E., Ferris N. P., Kitching R. P., Knowles N. J. 1999; Identification of neutralizing epitopes on a European strain of swine vesicular disease virus. Journal of General Virology 80:277–282
    [Google Scholar]
  25. Rebel J. M. J., Leendertse C. H., Dekker A., van Poelwijk F., Moormann R. J. M. 2000; Construction of a full-length infectious cDNA clone of swine vesicular disease virus strain NET/1/92 and analysis of new antigenic variants derived from it. Journal of General Virology 81:2763–2769
    [Google Scholar]
  26. Seechurn P., Knowles N. J., McCauley J. W. 1990; The complete nucleotide sequence of a pathogenic swine vesicular disease virus. Virus Research 16:255–274
    [Google Scholar]
  27. Tsuda T., Tokui T., Onodera T. 1987; Induction of neutralizing antibodies by structural proteins VP1 and VP2 of swine vesicular disease virus. Japanese Journal of Veterinary Science 49:129–132
    [Google Scholar]
  28. Usherwood E. J., Nash A. A. 1995; Lymphocyte recognition of picornaviruses. Journal of General Virology 76:499–508
    [Google Scholar]
  29. Zhang G., Haydon D. T., Knowles N. J., McCauley J. W. 1999; Molecular evolution of swine vesicular disease virus. Journal of General Virology 80:639–651
    [Google Scholar]
  30. Zhang G., Wilsden G., Knowles N. J., McCauley J. W. 1993; Complete nucleotide sequence of a coxsackie B5 virus and its relationship to swine vesicular disease virus. Journal of General Virology 74:845–853
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-1-35
Loading
/content/journal/jgv/10.1099/0022-1317-83-1-35
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error