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Volume 96, Issue 12

Genome analysis and gene identification of myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host Free

Abstract

The genome sequence, genetic characterization and gene function of myovirus isolated from Lake Dianchi in China (MaMV-DC) have been analysed. The genome DNA is 169 223 bp long, with 170 predicted protein-coding genes (–) and a tRNA gene. About one-sixth of these genes have homologues in the host cyanobacteria . The genome carries a gene homologous to host , which encodes a protein involved in the degradation of cyanobacterial phycobilisome. Its expression during MaMV-DC infection was confirmed by reverse transcriptase PCR and Western blot detection and abundant expression was companied by the significant decline of phycocyanin content and massive release of progeny MaMV-DC. In addition, expressing MaMV-DC reduced the phycocyanin peak and the phycocyanin to chlorophyll ratio in model cyanobacteria. These results confirm that horizontal gene transfer events have occurred between cyanobacterial host and cyanomyovirus and suggest that MaMV-DC carrying host-derived genes (such as , that codes for NblA) is responsible for more efficient expression of cyanophage genes and release of progeny cyanophage. This study provides novel insight into the horizontal gene transfer in cyanophage and the interactions between cyanophage and their host.

  • Received:
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© 2015 The Authors
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2015-12-01
2024-04-19
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Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host
J. Gen. Virol. 96, 3681 (2015); https://doi.org/10.1099/jgv.0.000290
/content/journal/jgv/10.1099/jgv.0.000290
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