RT Journal Article SR Electronic(1) A1 Do, Lien Anh Ha A1 Wilm, Andreas A1 van Doorn, H. Rogier A1 Lam, Ha Minh A1 Sim, Shuzhen A1 Sukumaran, Rashmi A1 Tran, Anh Tuan A1 Nguyen, Bach Hue A1 Tran, Thi Thu Loan A1 Tran, Quynh Huong A1 Vo, Quoc Bao A1 Dac, Nguyen Anh Tran A1 Trinh, Hong Nhien A1 Nguyen, Thi Thanh Hai A1 Binh, Bao Tinh Le A1 Le, Khanh A1 Nguyen, Minh Tien A1 Thai, Quang Tung A1 Vo, Thanh Vu A1 Ngo, Ngoc Quang Minh A1 Dang, Thi Kim Huyen A1 Cao, Ngoc Huong A1 Tran, Thu Van A1 Ho, Lu Viet A1 Farrar, Jeremy A1 de Jong, Menno A1 Chen, Swaine A1 Nagarajan, Niranjan A1 Bryant, Juliet E. A1 Hibberd, Martin L.YR 2015 T1 Direct whole-genome deep-sequencing of human respiratory syncytial virus A and B from Vietnamese children identifies distinct patterns of inter- and intra-host evolution JF Journal of General Virology, VO 96 IS 12 SP 3470 OP 3483 DO https://doi.org/10.1099/jgv.0.000298 PB Microbiology Society, SN 1465-2099, AB Human respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infections in children < 2 years of age. Little is known about RSV intra-host genetic diversity over the course of infection or about the immune pressures that drive RSV molecular evolution. We performed whole-genome deep-sequencing on 53 RSV-positive samples (37 RSV subgroup A and 16 RSV subgroup B) collected from the upper airways of hospitalized children in southern Vietnam over two consecutive seasons. RSV A NA1 and RSV B BA9 were the predominant genotypes found in our samples, consistent with other reports on global RSV circulation during the same period. For both RSV A and B, the M gene was the most conserved, confirming its potential as a target for novel therapeutics. The G gene was the most variable and was the only gene under detectable positive selection. Further, positively selected sites in G were found in close proximity to and in some cases overlapped with predicted glycosylation motifs, suggesting that selection on amino acid glycosylation may drive viral genetic diversity. We further identified hotspots and coldspots of intra-host genetic diversity in the RSV genome, some of which may highlight previously unknown regions of functional importance., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000298