RT Journal Article SR Electronic(1) A1 Denesvre, Caroline A1 Rémy, Sylvie A1 Trapp-Fragnet, Laetitia A1 Smith, Lorraine P. A1 Georgeault, Sonia A1 Vautherot, Jean-François A1 Nair, VenugopalYR 2016 T1 Marek's disease virus undergoes complete morphogenesis after reactivation in a T-lymphoblastoid cell line transformed by recombinant fluorescent marker virus JF Journal of General Virology, VO 97 IS 2 SP 480 OP 486 DO https://doi.org/10.1099/jgv.0.000354 PB Microbiology Society, SN 1465-2099, AB T-lymphocytes are central targets of Marek's disease, a major chicken disease induced by the oncogenic alphaherpesvirus Marek's disease virus (MDV). T-lymphocyte infection is also associated with immunosuppression and virus latency. To decipher viral morphogenesis in T-lymphocytes, we used the recombinant vRB-1B 47EGFP marker virus to generate a new lymphoblastoid cell line, 3867K, that exhibited typical properties of other MDV-transformed chicken cell lines in term of cell markers, reactivation rate and infectivity. Examination of reactivating EGFP-positive 3867K cells by transmission electron microscopy revealed the presence of most types of herpesvirus particles inside the cells but no extracellular ones. Quantification of virion types indicated only 5 % cytoplasmic particles, with 0.5 % being mature. This study demonstrated that MDV morphogenesis is complete upon reactivation in T-lymphocytes, albeit with poor efficiency, with a defect in the exit of virions from the nucleus and secondary envelopment, as occurs in infected fibroblasts., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000354