@article{mbs:/content/journal/jgv/10.1099/jgv.0.000366, author = "Van Dung, Nguyen and Anh, Pham Hong and Van Cuong, Nguyen and Hoa, Ngo Thi and Carrique-Mas, Juan and Hien, Vo Be and Sharp, C. and Rabaa, M. and Berto, A. and Campbell, James and Baker, Stephen and Farrar, Jeremy and Woolhouse, Mark E. and Bryant, Juliet E. and Simmonds, Peter", title = "Large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in Vietnam", journal= "Journal of General Virology", year = "2016", volume = "97", number = "2", pages = "378-388", doi = "https://doi.org/10.1099/jgv.0.000366", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000366", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "A recent survey of pigs in Dong Thap province, Vietnam identified a high frequency of enterovirus species G (EV-G) infection (144/198; 72.7 %). Amongst these was a plethora of EV-G types (EV-G1, EV-G6 and four new types EV-G8–EV-G11). To better characterize the genetic diversity of EV-G and investigate the possible existence of further circulating types, we performed a larger-scale study on 484 pig and 45 farm-bred boar faecal samples collected in 2012 and 2014, respectively. All samples from the previous and current studies were also screened for kobuviruses. The overall EV infection frequency remained extremely high (395/484; 81.6 %), but with comparable detection rates and viral loads between healthy and diarrhoeic pigs; this contrasted with less frequent detection of EV-G in boars (4/45; 8.9 %). EV was most frequently detected in pigs ≤ 14 weeks old (∼95 %) and declined in older pigs. Infections with EV-G1 and EV-G6 were most frequent, whilst less commonly detected types included EV-G3, EV-G4 and EV-G8–EV-G11, and five new types (EV-G12–EV-G16). In contrast, kobuvirus infection frequency was significantly higher in diarrhoeic pigs (40.9 versus 27.6 %; P = 0.01). Kobuviruses also showed contrasting epizootiologies and age associations; a higher prevalence was found in boars (42 %) compared with domestic pigs (29 %), with the highest infection frequency amongst pigs >52 weeks old. Although genetically diverse, all kobuviruses identified belonged to the species Aichivirus C. In summary, this study confirms infection with EV-G was endemic in Vietnamese domestic pigs and exhibits high genetic diversity and extensive inter-type recombination.", }