RT Journal Article SR Electronic(1) A1 Colmant, Agathe M. G. A1 Bielefeldt-Ohmann, Helle A1 Hobson-Peters, Jody A1 Suen, Willy W. A1 O'Brien, Caitlin A. A1 van den Hurk, Andrew F. A1 Hall, Roy A.YR 2016 T1 A newly discovered flavivirus in the yellow fever virus group displays restricted replication in vertebrates JF Journal of General Virology, VO 97 IS 5 SP 1087 OP 1093 DO https://doi.org/10.1099/jgv.0.000430 PB Microbiology Society, SN 1465-2099, AB A novel flavivirus, provisionally named Bamaga virus (BgV), was isolated from Culex annulirostris mosquitoes collected from northern Australia. Phylogenetic analysis of the complete nucleotide sequence of the BgV genome revealed it clustered with the yellow fever virus (YFV) group, and was most closely related to Edge Hill virus (EHV), another Australian flavivirus, with 61.9 % nucleotide and 63.7 % amino acid sequence identity. Antigenic analysis of the envelope and pre-membrane proteins of BgV further revealed epitopes common to EHV, dengue and other mosquito-borne flaviviruses. However, in contrast to these viruses, BgV displayed restricted growth in a range of vertebrate cell lines with no or relatively slow replication in inoculated cultures. There was also restricted BgV replication in virus-challenged mice. Our results indicate that BgV is an evolutionary divergent member of the YFV group of flaviviruses, and represents a novel system to study mechanisms of virus host-restriction and transmission., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000430