1887

Abstract

African swine fever (ASF) is an emerging disease threat for the swine industry worldwide. No ASF vaccine is available and progress is hindered by lack of knowledge concerning the extent of ASFV strain diversity and the viral antigens conferring type-specific protective immunity in pigs. Available data from vaccination/challenge experiments in pigs indicate that ASF protective immunity may be haemadsorption inhibition (HAI) serotype-specific. Recently, we have shown that two ASFV proteins, CD2v (EP402R) and C-type lectin (EP153R), are necessary and sufficient for mediating HAI serological specificity (Malogolovkin , 2015).. Here, using ASFV inter-serotypic chimeric viruses and vaccination/challenge experiments in pigs, we demonstrate that serotype-specific CD2v and/or C-type lectin proteins are important for protection against homologous ASFV infection. Thus, these viral proteins represent significant protective antigens for ASFV that should be targeted in future vaccine design and development. Additionally, these data support the concept of HAI serotype-specific protective immunity.

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2016-07-01
2024-03-29
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References

  1. Argilaguet J. M., Pérez-Martín E., Nofrarías M., Gallardo C., Accensi F., Lacasta A., Mora M., Ballester M., Galindo-Cardiel I. et al. 2012; DNA vaccination partially protects against African swine fever virus lethal challenge in the absence of antibodies. PLoS One 7:e40942 [View Article][PubMed]
    [Google Scholar]
  2. Argilaguet J. M., Pérez-Martín E., López S., Goethe M., Escribano J. M., Giesow K., Keil G. M., Rodríguez F. 2013; BacMam immunization partially protects pigs against sublethal challenge with African swine fever virus. Antiviral Res 98:61–65 [View Article][PubMed]
    [Google Scholar]
  3. Balyshev V. M., Fedorishhev I. V., Salina M. V. 1995; Study of serotype interactions of ASF virus strains both in vitro and in vivo (Russian). In Virusniye Bolezni Zhivotnikh [Virus diseases of animals] , p. 230 Vladimir: ARRIAH;
    [Google Scholar]
  4. Balyshev V. M., Kalantaenko J. F., Zhoukov A. N. 2010; Immunobiological and molecular-genetic characteristics of ASF virus isolates detected in the RF (Russian). In Orientirovannyie I Fundamentalniye Issledovanija I Ih Realizatsija v Agropromishlennom Compekse Rossii: Materiali Vserossiyskoy Nauchnoi Konferentsii , pp. 94–98 [Basic and targeted research in Russian agrocluster: Proceedings of all-Russian scientific conference] Moscow: Russian Acadamy of Agriculture Science;
    [Google Scholar]
  5. Barderas M. G., Rodríguez F., Gómez-Puertas P., Avilés M., Beitia F., Alonso C., Escribano J. M. 2001; Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins. Arch Virol 146:1681–1691[PubMed] [CrossRef]
    [Google Scholar]
  6. Boinas F. S., Hutchings G. H., Dixon L. K., Wilkinson P. J. 2004; Characterization of pathogenic and non-pathogenic African swine fever virus isolates from Ornithodoros erraticus inhabiting pig premises in Portugal. J Gen Virol 85:2177–2187 [View Article][PubMed]
    [Google Scholar]
  7. Coggins L. 1968; A modified hemadsorption-inhibition test for African swine fever virus. Bull Epizoot Dis Afr 16:61–64
    [Google Scholar]
  8. Coggins L. 1974; African swine fever virus. Pathogenesis. Prog Med Virol 18:48–63[PubMed]
    [Google Scholar]
  9. Cubillos C., Gómez-Sebastian S., Moreno N., Nuñez M. C., Mulumba-Mfumu L. K., Quembo C. J., Heath L., Etter E. M., Jori F. et al. 2013; African swine fever virus serodiagnosis: a general review with a focus on the analyses of African serum samples. Virus Res 173:159–167 [View Article][PubMed]
    [Google Scholar]
  10. Detray D. E. 1957; Persistence of viremia and immunity in African swine fever. Am J Vet Res 18:811–816[PubMed]
    [Google Scholar]
  11. Gallardo C., Soler A., Nieto R., Carrascosa A. L., De Mia G. M., Bishop R. P., Martins C., Fasina F. O., Couacy-Hymman E. et al. 2013; Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests. Vet Microbiol 162:32–43 [View Article][PubMed]
    [Google Scholar]
  12. Gogin A., Gerasimov V., Malogolovkin A., Kolbasov D. 2013; African swine fever in the North Caucasus region and the Russian Federation in years 2007–2012. Virus Res 173:198–203 [View Article][PubMed]
    [Google Scholar]
  13. Gómez-Puertas P., Rodríguez F., Oviedo J. M., Brun A., Alonso C., Escribano J. M. 1998; The African swine fever virus proteins p54 and p30 are involved in two distinct steps of virus attachment and both contribute to the antibody-mediated protective immune response. Virology 243:461–471 [View Article][PubMed]
    [Google Scholar]
  14. King D. P., Reid S. M., Hutchings G. H., Grierson S. S., Wilkinson P. J., Dixon L. K., Bastos A. D., Drew T. W. 2003; Development of a TaqMan PCR assay with internal amplification control for the detection of African swine fever virus. J Virol Methods 107:53–61[PubMed] [CrossRef]
    [Google Scholar]
  15. Knudsen R. C., Genovesi E. V., Whyard T. C. 1987; In vitro immune serum-mediated protection of pig monocytes against African swine fever virus. Am J Vet Res 48:1067–1071[PubMed]
    [Google Scholar]
  16. Lacasta A., Ballester M., Monteagudo P. L., Rodríguez J. M., Salas M. L., Accensi F., Pina-Pedrero S., Bensaid A., Argilaguet J. et al. 2014; Expression library immunization can confer protection against lethal challenge with African swine fever virus. J Virol 88:13322–13332 [View Article][PubMed]
    [Google Scholar]
  17. Leitão A., Cartaxeiro C., Coelho R., Cruz B., Parkhouse R. M., Portugal F., Vigário J. D., Martins C. L. 2001; The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response. J Gen Virol 82:513–523 [View Article][PubMed]
    [Google Scholar]
  18. Lewis T., Zsak L., Burrage T. G., Lu Z., Kutish G. F., Neilan J. G., Rock D. L. 2000; An African swine fever virus ERV1-ALR homologue, 9GL, affects virion maturation and viral growth in macrophages and viral virulence in swine. J Virol 74:1275–1285[PubMed] [CrossRef]
    [Google Scholar]
  19. Malmquist W. A. 1963; Serologic and immunologic studies with African swine fever virus. Am J Vet Res 24:450–459[PubMed]
    [Google Scholar]
  20. Malogolovkin A., Burmakina G., Titov I., Sereda A., Gogin A., Baryshnikova E., Kolbasov D. 2015a; Comparative analysis of African swine fever virus genotypes and serogroups. Emerg Infect Dis 21:312–315 [CrossRef]
    [Google Scholar]
  21. Malogolovkin A., Burmakina G., Tulman E. R., Delhon G., Diel D. G., Salnikov N., Kutish G. F., Kolbasov D., Rock D. L. 2015b; African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity. J Gen Virol 96:866–873 [CrossRef]
    [Google Scholar]
  22. Mebus C. A. 1988; African swine fever. Adv Virus Res 35:251–269[PubMed] [CrossRef]
    [Google Scholar]
  23. Montgomery R. E. 1921; On a form of swine fever occuring in British East Africa (Kenya Colony). J Comp Pathol 34:159–191 [CrossRef]
    [Google Scholar]
  24. Moore D. M., Zsak L., Neilan J. G., Lu Z., Rock D. L. 1998; The African swine fever virus thymidine kinase gene is required for efficient replication in swine macrophages and for virulence in swine. J Virol 72:10310–10315[PubMed]
    [Google Scholar]
  25. Neilan J. G., Zsak L., Lu Z., Burrage T. G., Kutish G. F., Rock D. L. 2004; Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection. Virology 319:337–342 [View Article][PubMed]
    [Google Scholar]
  26. Oura C. A., Denyer M. S., Takamatsu H., Parkhouse R. M. 2005; In vivo depletion of CD8+ T lymphocytes abrogates protective immunity to African swine fever virus. J Gen Virol 86:2445–2450 [View Article][PubMed]
    [Google Scholar]
  27. Pan I. C., Trautman R., Hess W. R., DeBoer C. J., Tessler J., Ordas A., Botija C. S., Ovejero J., Sanchez M. C. 1974; African swine fever: comparison of four serotests on porcine serums in Spain. Am J Vet Res 35:787–790[PubMed]
    [Google Scholar]
  28. Portugal R., Coelho J., Höper D., Little N. S., Smithson C., Upton C., Martins C., Leitão A., Keil G. M. 2015; Related strains of African swine fever virus with different virulence: genome comparison and analysis. J Gen Virol 96:408–419 [View Article][PubMed]
    [Google Scholar]
  29. Rowlands R. J., Duarte M. M., Boinas F., Hutchings G., Dixon L. K. 2009; The CD2v protein enhances African swine fever virus replication in the tick vector, Ornithodoros erraticus . Virology 393:319–328 [View Article][PubMed]
    [Google Scholar]
  30. Ruiz Gonzalvo, F., Carnero M. E., Bruyel V. 1981; Immunological responses of pigs to partially attenuated ASF and their resistance to virulent homologous and heterologous viruses. In FAO/CEC Expert Consultation in ASF Research , pp. 206–216 Edited by Wilkinson P. J. Rome:
    [Google Scholar]
  31. Ruiz Gonzalvo F., Carnero M. E., Caballero C., Martínez J. 1986; Inhibition of African swine fever infection in the presence of immune sera in vivo and in vitro . Am J Vet Res 47:1249–1252[PubMed]
    [Google Scholar]
  32. Ruíz-Gonzalvo F., Coll J. M. 1993; Characterization of a soluble hemagglutinin induced in African swine fever virus-infected cells. Virology 196:769–777 [View Article][PubMed]
    [Google Scholar]
  33. Ruiz-Gonzalvo F., Rodríguez F., Escribano J. M. 1996; Functional and immunological properties of the baculovirus-expressed hemagglutinin of African swine fever virus. Virology 218:285–289 [View Article][PubMed]
    [Google Scholar]
  34. Sereda A. D., Solovkin S. L., Fugina L. G., Makarov V. V. 1992; [Immune reactions to the African swine fever virus] (Russian). Vopr Virusol 37:168–170[PubMed]
    [Google Scholar]
  35. Sereda A. D., Balyshev V. M. 2011; [Antigenic diversity of African swine fever viruses] (Russian). Vopr Virusol 56:38–42[PubMed]
    [Google Scholar]
  36. Sánchez-Vizcaíno J. M., Mur L., Martínez-López B. 2013; African swine fever (ASF): five years around Europe. Vet Microbiol 165:45–50 [View Article][PubMed]
    [Google Scholar]
  37. Vigário J. D., Terrinha A. M., Bastos A. L., Moura-Nunes J. F., Marques D., Silva J. F. 1970; Serological behaviour of isolated African swine fever virus. Brief report. Archiv Gesamte Virusforschung 31:387–389 [View Article][PubMed]
    [Google Scholar]
  38. Vigário J. D., Terrinha A. M., Moura Nunes J. F. 1974; Antigenic relationships among strains of African swine fecre virus. Archiv Gesamte Virusforschung 45:272–277 [View Article][PubMed]
    [Google Scholar]
  39. Vishnjakov I., Mitin N., Karpov G., Kurinnov V., Jashin A. 1991; Differentiation African and classical swine fever viruses. Veterinariya 4:28–31
    [Google Scholar]
  40. Vishnjakov I. F., Mitin N. I., Petrov J. I. 1995; Seroimmunological classification of African swine fever virus natural isolates. In Actualniye Voprosi Veterinarnoy Virusologii Materiali Naucho-Practicheskoy Conferentsii VNIIVViM “Klassicheskaya Chuma Sviney Neotlozhniye Problemi Nauki a Praktiki” , pp. 141–143 [Rational question of veterinary virology. Proceedings of scientific conference at VNIIVViM: classical swine fever – urgent issues of science and practice] Pokrov: VNIVViM;
    [Google Scholar]
  41. Zsak L., Lu Z., Kutish G. F., Neilan J. G., Rock D. L. 1996; An African swine fever virus virulence-associated gene NL-S with similarity to the herpes simplex virus ICP34.5 gene. J Virol 70:8865–8871[PubMed]
    [Google Scholar]
  42. Zsak L., Caler E., Lu Z., Kutish G. F., Neilan J. G., Rock D. L. 1998; A nonessential African swine fever virus gene UK is a significant virulence determinant in domestic swine. J Virol 72:1028–1035[PubMed]
    [Google Scholar]
  43. Zsak L., Burrage T. G., Neilan J. G., Lu Z., Kutish G., Rock D. L. 2010; African swine fever virus CD2-like protein mediates monocyte infection-inhibition. In XVII International Poxvirus, Asfivirus, and Iridovirus SymposiumJune 5–10 , p. 7.19 Sedona, AZ:
    [Google Scholar]
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