@article{mbs:/content/journal/jgv/10.1099/jgv.0.000684, author = "Iwata, Kento and Haas-Stapleton, Eric and Kunimi, Yasuhisa and Inoue, Maki N and Nakai, Madoka", title = "Midgut-based resistance to oral infection by a nucleopolyhedrovirus in the laboratory-selected strain of the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae)", journal= "Journal of General Virology", year = "2017", volume = "98", number = "2", pages = "296-304", doi = "https://doi.org/10.1099/jgv.0.000684", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000684", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "resistance", keywords = "baculovirus", keywords = "midgut", keywords = "Adoxophyes honmai", keywords = "nucleopolyhedrovirus", abstract = "A strain of Adoxophyes honmai resistant to Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) was established from a field-collected colony by repeated selection. Fifth-instar larvae of this resistant strain (R-strain) had over 66 666-fold greater resistance in terms of 50 % lethal concentration values to oral infection of AdhoNPV than non-selected strain larvae (susceptible for AdhoNPV; S2-strain). In this study, the mechanism of resistance to AdhoNPV was determined in R-strain larvae. An assessment of viral genome replication in AdhoNPV-infected S2- and R-strain larvae by quantitative PCR showed no viral genome replication occurring in R-strain larvae. Transcription of AdhoNPV ie-1, vp39 and polyhedrin genes was also not detected in R-strain midgut cells. Besides, a fluorescent brightener had no effect on AdhoNPV infection in either S2- or R-strain. However, binding and fusion of occlusion-derived virus with R-strain were significantly lower than those of S2-strain. These findings suggest that R-strain Adoxophyes honmai larvae possess a midgut-based resistance to oral infection by AdhoNPV in which midgut epithelial cells are infected less efficiently.", }