RT Journal Article SR Electronic(1) A1 Esona, Mathew D A1 Roy, Sunando A1 Rungsrisuriyachai, Kunchala A1 Sanchez, Jacqueline A1 Vasquez, Lina A1 Gomez, Virgen A1 Rios, Lourdes Aviles A1 Bowen, Michael D A1 Vazquez, MariettaYR 2017 T1 Characterization of a triple-recombinant, reassortant rotavirus strain from the Dominican Republic JF Journal of General Virology, VO 98 IS 2 SP 134 OP 142 DO https://doi.org/10.1099/jgv.0.000688 PB Microbiology Society, SN 1465-2099, AB We report the genome of a novel human triple-recombinant G4P[6–8_R] mono-reassortant strain identified in a stool sample from the Dominican Republic during routine facility-based rotavirus strain surveillance. The strain was designated as RVA/Human-wt/DOM/2013840364/2013/G4P[6–8_R], with a genomic constellation of G4-P[6–8_R]-I1-R1-C1-M1-(A1-A8_R)-N1-(T1-T7_R)-E1-H1. Recombinant gene segments NSP1 and NSP3 were generated as a result of recombination between genogroup 1 rotavirus A1 human strain and a genotype A8 porcine strain and between genogroup 1 rotavirus T1 human strain and a genotype T7 bovine strain, respectively. Analyses of the RNA secondary structures of gene segment VP4, NSP1 and NSP3 showed that all the recombinant regions appear to start in a loop (single-stranded) region and terminate in a stem (double-stranded) structure. Also, the VP7 gene occupied lineage VII within the G4 genotypes consisting of mostly porcine or porcine-like G4 strains, suggesting the occurrence of reassortment. The remaining gene segments clustered phylogenetically with genogroup 1 strains. This exchange of whole or partial genetic materials between rotaviruses by recombination and reassortment contributes directly to their diversification, adaptation and evolution., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000688