%0 Journal Article %A Hassan, Ahmed O %A Vemula, Sai V %A Sharma, Anurag %A Bangari, Dinesh S %A Mishra, Krishna K %A Mittal, Suresh K %T 155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication %D 2017 %J Journal of General Virology, %V 98 %N 4 %P 749-753 %@ 1465-2099 %R https://doi.org/10.1099/jgv.0.000707 %K bovine adenovirus type 3 %K adenoviral novel structural protein %K 155R protein %I Microbiology Society, %X Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000707