155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication
Hassan, Ahmed O and Vemula, Sai V and Sharma, Anurag and Bangari, Dinesh S and Mishra, Krishna K and Mittal, Suresh K,, 98, 749-753 (2017),
doi = https://doi.org/10.1099/jgv.0.000707,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions.,
language=,
type=