1887

Abstract

Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions.

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2017-04-01
2024-03-29
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References

  1. Vemula SV, Mittal SK. Production of adenovirus vectors and their use as a delivery system for influenza vaccines. Expert Opin Biol Ther 2010; 10:1469–1487 [View Article]
    [Google Scholar]
  2. Sharma A, Tandon M, Bangari DS, Mittal SK. Adenoviral vector-based strategies for cancer therapy. Curr Drug ther 2009; 4:117–138 [View Article][PubMed]
    [Google Scholar]
  3. Vellinga J, van der Heijdt S, Hoeben RC. The adenovirus capsid: major progress in minor proteins. J Gen Virol 2005; 86:1581–1588 [View Article][PubMed]
    [Google Scholar]
  4. Reddy VS, Nemerow GR. Structures and organization of adenovirus cement proteins provide insights into the role of capsid maturation in virus entry and infection. Proc Natl Acad Sci USA 2014; 111:11715–11720 [View Article][PubMed]
    [Google Scholar]
  5. Ahi YS, Vemula SV, Mittal SK. Adenoviral E2 IVa2 protein interacts with L4 33K protein and E2 DNA-binding protein. J Gen Virol 2013; 94:1325–1334 [View Article][PubMed]
    [Google Scholar]
  6. Mattson DE. Naturally occurring infection of calves with a bovine adenovirus. Am J Vet Res 1973; 34:623–629[PubMed]
    [Google Scholar]
  7. Mittal SK, Tikoo SK, van Donkersgoed J, Beskorwayne T, Godson DL et al. Experimental inoculation of heifers with bovine adenovirus type 3. Can J Vet Res 1999; 63:153–156[PubMed]
    [Google Scholar]
  8. Harrach B, Benko M, Both GW, Brown M, Davison AJ et al. The double stranded DNA viruses: Adenoviridae. In King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. (editors) Virus Taxonomy: Classification and Nomenclature of Viruses. Ninth Report of the International Committee on Taxonomy of Viruses Waltham: Academic Press - Elsevier; 2011 pp. 125–141
    [Google Scholar]
  9. Reddy PS, Idamakanti N, Zakhartchouk AN, Baxi MK, Lee JB et al. Nucleotide sequence, genome organization, and transcription map of bovine adenovirus type 3. J Virol 1998; 72:1394–1402[PubMed]
    [Google Scholar]
  10. Mittal SK, Prevec L, Babiuk LA, Graham FL. Sequence analysis of bovine adenovirus type 3 early region 3 and fibre protein genes. J Gen Virol 1992; 73:3295–3300 [View Article][PubMed]
    [Google Scholar]
  11. Ruigrok RW, Barge A, Mittal SK, Jacrot B. The fibre of bovine adenovirus type 3 is very long but bent. J Gen Virol 1994; 75:2069–2073 [View Article][PubMed]
    [Google Scholar]
  12. Zheng B, Mittal SK, Graham FL, Prevec L. The E1 sequence of bovine adenovirus type 3 and complementation of human adenovirus type 5 E1A function in bovine cells. Virus Res 1994; 31:163–186 [View Article][PubMed]
    [Google Scholar]
  13. Reddy PS, Idamakanti N, Chen Y, Whale T, Babiuk LA et al. Replication-defective bovine adenovirus type 3 as an expression vector. J Virol 1999; 73:9137–9144[PubMed]
    [Google Scholar]
  14. van Olphen AL, Tikoo SK, Mittal SK. Characterization of bovine adenovirus type 3 E1 proteins and isolation of E1-expressing cell lines. Virology 2002; 295:108–118 [View Article][PubMed]
    [Google Scholar]
  15. Singh N, Pandey A, Jayashankar L, Mittal SK. Bovine adenoviral vector-based H5N1 influenza vaccine overcomes exceptionally high levels of pre-existing immunity against human adenovirus. Mol Ther 2008; 16:965–971 [View Article][PubMed]
    [Google Scholar]
  16. Sharma A, Tandon M, Ahi YS, Bangari DS, Vemulapalli R et al. Evaluation of cross-reactive cell-mediated immune responses among human, bovine and porcine adenoviruses. Gene Ther 2010; 17:634–642 [View Article][PubMed]
    [Google Scholar]
  17. Bangari DS, Shukla S, Mittal SK. Comparative transduction efficiencies of human and nonhuman adenoviral vectors in human, murine, bovine, and porcine cells in culture. Biochem Biophys Res Commun 2005; 327:960–966 [View Article][PubMed]
    [Google Scholar]
  18. Sharma A, Bangari DS, Tandon M, Pandey A, Hogenesch H et al. Comparative analysis of vector biodistribution, persistence and gene expression following intravenous delivery of bovine, porcine and human adenoviral vectors in a mouse model. Virology 2009; 386:44–54 [View Article][PubMed]
    [Google Scholar]
  19. Bangari DS, Sharma A, Mittal SK. Bovine adenovirus type 3 internalization is independent of primary receptors of human adenovirus type 5 and porcine adenovirus type 3. Biochem Biophys Res Commun 2005; 331:1478–1484 [View Article][PubMed]
    [Google Scholar]
  20. Li X, Bangari DS, Sharma A, Mittal SK. Bovine adenovirus serotype 3 utilizes sialic acid as a cellular receptor for virus entry. Virology 2009; 392:162–168 [View Article][PubMed]
    [Google Scholar]
  21. Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res 1981; 9:5233–5252 [View Article][PubMed]
    [Google Scholar]
  22. Nishikawa T, Ota T, Isogai T. Prediction whether a human cDNA sequence contains initiation codon by combining statistical information and similarity with protein sequences. Bioinformatics 2000; 16:960–967 [View Article][PubMed]
    [Google Scholar]
  23. Ceroni A, Passerini A, Vullo A, Frasconi P. DISULFIND: a disulfide bonding state and cysteine connectivity prediction server. Nucleic Acids Res 2006; 34:W177–W181 [View Article][PubMed]
    [Google Scholar]
  24. Petersen TN, Brunak S, von Heijne G, Nielsen H. SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods 2011; 8:785–786 [View Article][PubMed]
    [Google Scholar]
  25. Mitchell A, Chang HY, Daugherty L, Fraser M, Hunter S et al. The InterPro protein families database: the classification resource after 15 years. Nucleic Acids Res 2015; 43:D213–D221 [View Article][PubMed]
    [Google Scholar]
  26. Gupta R, Jung E, Brunak S. 2004; Prediction of N-glycosylation sites in human proteins. www.cbs.dtu.dk/services/NetNGlyc/
  27. Steentoft C, Vakhrushev SY, Joshi HJ, Kong Y, Vester-Christensen MB et al. Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology. EMBO J 2013; 32:1478–1488 [View Article][PubMed]
    [Google Scholar]
  28. van Olphen AL, Mittal SK. Development and characterization of bovine x human hybrid cell lines that efficiently support the replication of both wild-type bovine and human adenoviruses and those with E1 deleted. J Virol 2002; 76:5882–5892 [View Article][PubMed]
    [Google Scholar]
  29. Ahi YS, Vemula SV, Hassan AO, Costakes G, Stauffacher C et al. Adenoviral L4 33K forms ring-like oligomers and stimulates ATPase activity of IVa2: implications in viral genome packaging. Front Microbiol 2015; 6:318 [View Article][PubMed]
    [Google Scholar]
  30. van Olphen AL, Mittal SK. Generation of infectious genome of bovine adenovirus type 3 by homologous recombination in bacteria. J Virol Methods 1999; 77:125–129 [View Article][PubMed]
    [Google Scholar]
  31. Vellinga J, van den Wollenberg DJ, van der Heijdt S, Rabelink MJ, Hoeben RC. The coiled-coil domain of the adenovirus type 5 protein IX is dispensable for capsid incorporation and thermostability. J Virol 2005; 79:3206–3210 [View Article][PubMed]
    [Google Scholar]
  32. Falgout B, Ketner G. Characterization of adenovirus particles made by deletion mutants lacking the fiber gene. J Virol 1988; 62:622–625[PubMed]
    [Google Scholar]
  33. Krasnykh VN, Mikheeva GV, Douglas JT, Curiel DT. Generation of recombinant adenovirus vectors with modified fibers for altering viral tropism. J Virol 1996; 70:6839–6846[PubMed]
    [Google Scholar]
  34. Magnusson MK, Hong SS, Boulanger P, Lindholm L. Genetic retargeting of adenovirus: novel strategy employing "deknobbing" of the fiber. J Virol 2001; 75:7280–7289 [View Article][PubMed]
    [Google Scholar]
  35. Mittal SK, Middleton DM, Tikoo SK, Babiuk LA. Pathogenesis and immunogenicity of bovine adenovirus type 3 in cotton rats (Sigmodon hispidus). Virology 1995; 213:131–139 [View Article][PubMed]
    [Google Scholar]
  36. Mittal SK, Middleton DM, Tikoo SK, Prevec L, Graham FL et al. Pathology and immunogenicity in the cotton rat (Sigmodon hispidus) model after infection with a bovine adenovirus type 3 recombinant virus expressing the firefly luciferase gene. J Gen Virol 1996; 77:1–9 [View Article][PubMed]
    [Google Scholar]
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