@article{mbs:/content/journal/jgv/10.1099/jgv.0.000711, author = "Lima, Diane A and Cibulski, Samuel P and Finkler, Fabrine and Teixeira, Thais F and Varela, Ana Paula M and Cerva, Cristine and Loiko, Márcia R and Scheffer, Camila M and dos Santos, Helton F and Mayer, Fabiana Q and Roehe, Paulo M", title = "Faecal virome of healthy chickens reveals a large diversity of the eukaryote viral community, including novel circular ssDNA viruses", journal= "Journal of General Virology", year = "2017", volume = "98", number = "4", pages = "690-703", doi = "https://doi.org/10.1099/jgv.0.000711", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000711", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "high-throughput sequencing", keywords = "broilers", keywords = "fecal", keywords = "virome", abstract = "This study is focused on the identification of the faecal virome of healthy chickens raised in high-density, export-driven poultry farms in Brazil. Following high-throughput sequencing, a total of 7743 de novo-assembled contigs were constructed and compared with known nucleotide/amino acid sequences from the GenBank database. Analyses with blastx revealed that 279 contigs (4 %) were related to sequences of eukaryotic viruses. Viral genome sequences (total or partial) indicative of members of recognized viral families, including Adenoviridae, Caliciviridae, Circoviridae, Parvoviridae, Picobirnaviridae, Picornaviridae and Reoviridae, were identified, some of those representing novel genotypes. In addition, a range of circular replication-associated protein encoding DNA viruses were also identified. The characterization of the faecal virome of healthy chickens described here not only provides a description of the viruses encountered in such niche but should also represent a baseline for future studies comparing viral populations in healthy and diseased chicken flocks. Moreover, it may also be relevant for human health, since chickens represent a significant proportion of the animal protein consumed worldwide.", }