1887

Abstract

Two goose megriviruses (W18 and HN56) were detected and sequenced. Both viruses possessed megrivirus-like genomic features, including unusually long genomes (9840 and 10 101 nt). W18 and HN56 were very similar to duck megrivirus (DMV) in the P2 and P3 regions, but much less similar in the P1 and 2A1 regions. HN56 may be a potential recombinant virus, with a distinct P1 region possibly originating from an unknown picornavirus. W18 may represent a novel type of DMV, showing a P1 sequence identity of 67 %. Similar levels (64–68 %) of P1 sequence identity were also displayed by melegrivirus A with W18 and DMV, demonstrating an equal genetic separation of the capsid region among W18, DMV and melegrivirus A. For the 2A1 region, the divergence among W18, HN56 and DMV was remarkable, involving point mutations and a long insertion/deletion. The present work contributes to the understanding of unique features and phylogeny of megriviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000720
2017-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/98/4/607.html?itemId=/content/journal/jgv/10.1099/jgv.0.000720&mimeType=html&fmt=ahah

References

  1. Adams MJ, King AM, Carstens EB. Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2013). Arch Virol 2013; 158:2023–2030 [View Article][PubMed]
    [Google Scholar]
  2. Boros Á, Pankovics P, Knowles NJ, Nemes C, Delwart E et al. Comparative complete genome analysis of chicken and turkey megriviruses (family Picornaviridae): long 3′ untranslated regions with a potential second open reading frame and evidence for possible recombination. J Virol 2014; 88:6434–6443 [View Article][PubMed]
    [Google Scholar]
  3. Honkavuori KS, Shivaprasad HL, Briese T, Street C, Hirschberg DL et al. Novel picornavirus in turkey poults with hepatitis, California, USA. Emerg Infect Dis 2011; 17:480–487 [View Article][PubMed]
    [Google Scholar]
  4. Hughes PJ, Stanway G. The 2A proteins of three diverse picornaviruses are related to each other and to the H-rev107 family of proteins involved in the control of cell proliferation. J Gen Virol 2000; 81:201–207 [View Article][PubMed]
    [Google Scholar]
  5. Kim HR, Yoon SJ, Lee HS, Kwon YK. Identification of a picornavirus from chickens with transmissible viral proventriculitis using metagenomic analysis. Arch Virol 2015; 160:701–709 [View Article][PubMed]
    [Google Scholar]
  6. Lau SK, Woo PC, Yip CC, Li KS, Fan RY et al. Chickens host diverse picornaviruses originated from potential interspecies transmission with recombination. J Gen Virol 2014; 95:1929–1944 [View Article][PubMed]
    [Google Scholar]
  7. Liao Q, Zheng L, Yuan Y, Shi J, Zhang D. Genomic characterization of a novel picornavirus in Pekin ducks. Vet Microbiol 2014; 172:78–91 [View Article][PubMed]
    [Google Scholar]
  8. Phan TG, Vo NP, Boros Á, Pankovics P, Reuter G et al. The viruses of wild pigeon droppings. PLoS One 2013; 8:e72787 [View Article][PubMed]
    [Google Scholar]
  9. Farkas T, Fey B, Hargitt E 3rd, Parcells M, Ladman B et al. Molecular detection of novel picornaviruses in chickens and turkeys. Virus Genes 2012; 44:262–272 [View Article][PubMed]
    [Google Scholar]
  10. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA 2005; 102:12891–12896 [View Article][PubMed]
    [Google Scholar]
  11. Sambrook J, Russell DW. Molecular Cloning: a Laboratory Manual, 3rd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 2001
    [Google Scholar]
  12. Boros Á, Pankovics P, Adonyi Á, Fenyvesi H, Day JM et al. A diarrheic chicken simultaneously co-infected with multiple picornaviruses: complete genome analysis of avian picornaviruses representing up to six genera. Virology 2016; 489:63–74 [View Article][PubMed]
    [Google Scholar]
  13. Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK et al. CDD: a conserved domain database for the functional annotation of proteins. Nucleic Acids Res 2011; 39:D225–D229 [View Article][PubMed]
    [Google Scholar]
  14. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  15. Knowles NJ, Hovi T, Hyypiä T, King AMQ, Lindberg AM et al. Picornaviridae. In King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. (editors) Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses San Diego: Elsevier Academic Press; 2012 pp. 855–880
    [Google Scholar]
  16. Snoeyenbos GH, Basch HI, Sevoian M. An infectious agent producing hepatitis in turkeys. Avian Dis 1959; 3:377–388 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000720
Loading
/content/journal/jgv/10.1099/jgv.0.000720
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error