RT Journal Article SR Electronic(1) A1 Nunes-Neto, Joaquim Pinto A1 Souza, William Marciel de A1 Acrani, Gustavo Olszanski A1 Romeiro, Marilia Farignoli A1 Fumagalli, MarcílioJorge A1 Vieira, Luiz Carlos A1 Medeiros, Daniele Barbosa de Almeida A1 Lima, Juliana Abreu A1 Lima, Clayton Pereira Silva de A1 Cardoso, Jedson Ferreira A1 Figueiredo, Luiz Tadeu Moraes A1 Silva, Sandro Patroca da A1 Tesh, Robert A1 Nunes, Márcio Roberto Teixeira A1 Vasconcelos, Pedro Fernando da CostaYR 2017 T1 Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil JF Journal of General Virology, VO 98 IS 4 SP 585 OP 594 DO https://doi.org/10.1099/jgv.0.000724 PB Microbiology Society, SN 1465-2099, AB The genus Phlebovirus includes the sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids of the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated. Here we describe the nearly complete sequences and genomic characterization of two phleboviruses belonging to the Bujaru antigenic complex: the prototype species and the Munguba virus. Furthermore, six previously unclassified phleboviruses isolated in Brazil were also sequenced and characterized: Ambe, Anhanga, Joa, Uriurana, Urucuri and Tapara viruses. The results of the phylogenetic analysis indicated that these viruses group with viruses of three antigenic complexes (Bujaru, Tapara and frijoles clades), with two unclassified phleboviruses. We also performed genomic reassortment analysis and confirmed that there were no events for the viruses described in this study, but we found a new potential reassortment in Medjerda Valley virus, which contains S and L segments of Arbia virus, and probably a unique M segment, both viruses circulate in the same geographic region, indicating these two isolates represent two distinct viruses. This study provides insights into the genetic diversity, classification and evolution of phleboviruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000724