RT Journal Article SR Electronic(1) A1 Kurosaki, Yohei A1 Ueda, Mahoko Takahashi A1 Nakano, Yusuke A1 Yasuda, Jiro A1 Koyanagi, Yoshio A1 Sato, Kei A1 Nakagawa, SoYR 2018 T1 Different effects of two mutations on the infectivity of Ebola virus glycoprotein in nine mammalian species JF Journal of General Virology, VO 99 IS 2 SP 181 OP 186 DO https://doi.org/10.1099/jgv.0.000999 PB Microbiology Society, SN 1465-2099, AB Ebola virus (EBOV), which belongs to the genus Ebolavirus, causes a severe and often fatal infection in primates, including humans, whereas Reston virus (RESTV) only causes lethal disease in non-human primates. Two amino acids (aa) at positions 82 and 544 of the EBOV glycoprotein (GP) are involved in determining viral infectivity. However, it remains unclear how these two aa residues affect the infectivity of Ebolavirus species in various hosts. Here we performed viral pseudotyping experiments with EBOV and RESTV GP derivatives in 10 cell lines from 9 mammalian species. We demonstrated that isoleucine at position 544/545 increases viral infectivity in all host species, whereas valine at position 82/83 modulates viral infectivity, depending on the viral and host species. Structural modelling suggested that the former residue affects viral fusion, whereas the latter residue influences the interaction with the viral entry receptor, Niemann–Pick C1., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000999