1887

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Volume 99, Issue 3

Chicken anaemia virus evades host immune responses in transformed lymphocytes Free

Abstract

Chicken anaemia virus (CAV) is a lymphotropic virus that causes anaemia and immunosuppression in chickens. Previously, we proposed that CAV evades host antiviral responses by disrupting T-cell signalling, but the precise cellular targets and modes of action remain elusive. In this study, we examined gene expression in Marek’s disease virus-transformed chicken T-cell line MSB-1 after infection with CAV using both a custom 5K immune-focused microarray and quantitative real-time PCR at 24, 48 and 72 h post-infection. The data demonstrate an intricate equilibrium between CAV and the host gene expression, displaying subtle but significant modulation of transcripts involved in the T-cell, inflammation and NF-κB signalling cascades. CAV efficiently blocked the induction of type-I interferons and interferon-stimulated genes at 72 h. The cell expression pattern implies that CAV subverts host antiviral responses and that the transformed environment of MSB-1 cells offers an opportunistic advantage for virus growth.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Chicken anaemia virus , immune evasion , microarray and T-lymphocytes
© 2018 The Authors
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2018-03-01
2024-04-19
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Chicken anaemia virus evades host immune responses in transformed lymphocytes
J. Gen. Virol. 99, 321 (2018); https://doi.org/10.1099/jgv.0.001011
/content/journal/jgv/10.1099/jgv.0.001011
/content/journal/jgv/10.1099/jgv.0.001011
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