Identification of a newly described OsHV-1 µvar from the North Adriatic Sea (Italy)
Abbadi, Miriam and Zamperin, Gianpiero and Gastaldelli, Michele and Pascoli, Francesco and Rosani, Umberto and Milani, Adelaide and Schivo, Alessia and Rossetti, Emanuele and Turolla, Edoardo and Gennari, Lorenzo and Toffan, Anna and Arcangeli, Giuseppe and Venier, Paola,, 99, 693-703 (2018),
doi = https://doi.org/10.1099/jgv.0.001042,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= The surveillance activities for abnormal bivalve mortality events in Italy include the diagnosis of ostreid herpesvirus type 1 (OsHV-1) in symptomatic oysters. OsHV-1-positive oysters (Crassostrea gigas) were used as a source for in vivo virus propagation and a virus-rich sample was selected to perform shotgun sequencing based on Illumina technology. Starting from this unpurified supernatant sample from gills and mantle, we generated 3.5 million reads (2×300 bp) and de novo assembled the whole genome of an Italian OsHV-1 microvariant (OsHV-1-PT). The OsHV-1-PT genome encodes 125 putative ORFs, 7 of which had not previously been predicted in other sequenced Malacoherpesviridae. Overall, OsHV-1-PT displays typical microvariant OsHV-1 genome features, while few polymorphisms (0.08 %) determine its uniqueness. As little is known about the genetic determinants of OsHV-1 virulence, comparing complete OsHV-1 genomes supports a better understanding of the virus pathogenicity and provides new insights into virus–host interactions.,
language=,
type=