RT Journal Article SR Electronic(1) A1 Abbadi, Miriam A1 Zamperin, Gianpiero A1 Gastaldelli, Michele A1 Pascoli, Francesco A1 Rosani, Umberto A1 Milani, Adelaide A1 Schivo, Alessia A1 Rossetti, Emanuele A1 Turolla, Edoardo A1 Gennari, Lorenzo A1 Toffan, Anna A1 Arcangeli, Giuseppe A1 Venier, PaolaYR 2018 T1 Identification of a newly described OsHV-1 µvar from the North Adriatic Sea (Italy) JF Journal of General Virology, VO 99 IS 5 SP 693 OP 703 DO https://doi.org/10.1099/jgv.0.001042 PB Microbiology Society, SN 1465-2099, AB The surveillance activities for abnormal bivalve mortality events in Italy include the diagnosis of ostreid herpesvirus type 1 (OsHV-1) in symptomatic oysters. OsHV-1-positive oysters (Crassostrea gigas) were used as a source for in vivo virus propagation and a virus-rich sample was selected to perform shotgun sequencing based on Illumina technology. Starting from this unpurified supernatant sample from gills and mantle, we generated 3.5 million reads (2×300 bp) and de novo assembled the whole genome of an Italian OsHV-1 microvariant (OsHV-1-PT). The OsHV-1-PT genome encodes 125 putative ORFs, 7 of which had not previously been predicted in other sequenced Malacoherpesviridae. Overall, OsHV-1-PT displays typical microvariant OsHV-1 genome features, while few polymorphisms (0.08 %) determine its uniqueness. As little is known about the genetic determinants of OsHV-1 virulence, comparing complete OsHV-1 genomes supports a better understanding of the virus pathogenicity and provides new insights into virus–host interactions., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001042