@article{mbs:/content/journal/jgv/10.1099/jgv.0.001074, author = "Bonnin, Ariane and Danneels, Adeline and Dubuisson, Jean and Goffard, Anne and Belouzard, Sandrine", title = "HCoV-229E spike protein fusion activation by trypsin-like serine proteases is mediated by proteolytic processing in the S2′ region", journal= "Journal of General Virology", year = "2018", volume = "99", number = "7", pages = "908-912", doi = "https://doi.org/10.1099/jgv.0.001074", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001074", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "spike protein", keywords = "entry", keywords = "HCoV-229E", keywords = "fusion", abstract = "Human coronavirus 229E (HCoV-229E) is responsible for common colds. Like other coronaviruses, HCoV-229E exploits cellular proteases to activate fusion mediated by the spike protein. We analysed the proteolytic processing of the HCoV-229E spike protein by trypsin-like serine proteases leading to activation of the fusion process. Unlike in other coronaviruses, HCoV-229E fusion activation appears to be a one-step process. Indeed, cleavage of the S1/S2 interface does not seem to be a prerequisite, and the fusion activation is highly reliant on the S2′ region, with arginine residue 683 acting as the recognition site.", }