HCoV-229E spike protein fusion activation by trypsin-like serine proteases is mediated by proteolytic processing in the S2′ region Bonnin, Ariane and Danneels, Adeline and Dubuisson, Jean and Goffard, Anne and Belouzard, Sandrine,, 99, 908-912 (2018), doi = https://doi.org/10.1099/jgv.0.001074, publicationName = Microbiology Society, issn = 0022-1317, abstract= Human coronavirus 229E (HCoV-229E) is responsible for common colds. Like other coronaviruses, HCoV-229E exploits cellular proteases to activate fusion mediated by the spike protein. We analysed the proteolytic processing of the HCoV-229E spike protein by trypsin-like serine proteases leading to activation of the fusion process. Unlike in other coronaviruses, HCoV-229E fusion activation appears to be a one-step process. Indeed, cleavage of the S1/S2 interface does not seem to be a prerequisite, and the fusion activation is highly reliant on the S2′ region, with arginine residue 683 acting as the recognition site., language=, type=