RT Journal Article SR Electronic(1) A1 Pena, Lindomar José A1 Miranda Guarines, Klarissa A1 Duarte Silva, Anna Jéssica A1 Sales Leal, Lígia Rosa A1 Mendes Félix, Daniele A1 Silva, Adalúcia A1 de Oliveira, Sheilla Andrade A1 Junqueira Ayres, Constância Flávia A1 Júnior, Abelardo Silva A1 de Freitas, Antonio CarlosYR 2018 T1 In vitro and in vivo models for studying Zika virus biology JF Journal of General Virology, VO 99 IS 12 SP 1529 OP 1550 DO https://doi.org/10.1099/jgv.0.001153 PB Microbiology Society, SN 1465-2099, AB The emergence and rapid spread of Zika virus (ZIKV) in the Americas has prompted the development of in vitro and in vivo models to understand several aspects of ZIKV biology and boost the development of vaccines and antivirals. In vitro model studies include reverse genetics systems, two-dimensional (2D) cell models, such as primary cells and cell lines, and ex vivo three-dimensional (3D) models derived from skin, brain and placenta. While these models are cost-effective and allow rigorous control of experimental variables, they do not always recapitulate in vivo scenarios. Thus, a number of in vivo models have been developed, including mosquitoes (Aedes sp. and Culex sp.), embryonated chicken eggs, immunocompetent and immunodeficient mice strains, hamsters, guinea pigs, conventional swine and non-human primates. In this review, we summarize the main research systems that have been developed in recent years and discuss their advantages, limitations and main applications., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001153