1887

Abstract

The full-length genomes of two DENV-1 viruses isolated during the 2005–2006 dengue incidents in Brunei were sequenced. Twenty five primer sets were designed to amplify contiguous overlapping fragments of approximately 500–600 base pairs spanning the entire sequence of the genome. The amplified PCR products were sent to a commercial laboratory for sequencing and the nucleotides and the deduced amino acids were determined. Sequence analysis of the envelope gene at the nucleotide and amino acid levels between the two isolates showed 92 and 96 % identity, respectively. Comparison of the envelope gene sequences with 68 other DENV-1 viruses of known genotypes placed the two isolates into two different genotypic groups. Isolate DS06/210505 belongs to genotype V together with some of the recent isolates from India (2003) and older isolates from Singapore (1990) and Burma (1976), while isolate DS212/110306 was clustered in genotype IV with the prototype Nauru strain (1974) and with some of the recent isolates from Indonesia (2004) and the Philippines (2002, 2001). In the full-length genome analysis at the nucleotide level, isolate DS06/210505 showed 94 % identity to the French Guyana strain (1989) in genotype V while isolate DS212/110306 had 96 % identity to the Nauru Island strain (1974) in genotype IV. This work constitutes the first complete genetic characterization of not only Brunei DENV-1 virus isolates, but also the first strain from Borneo Island. This study was the first to report the isolation of dengue virus in the country.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.005306-0
2009-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/90/3/678.html?itemId=/content/journal/jgv/10.1099/vir.0.005306-0&mimeType=html&fmt=ahah

References

  1. Alcon S., Talarmin A., Debruyne M., Falconar A., Deubel V., Flammand M. 2002; Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40:376–381 [CrossRef]
    [Google Scholar]
  2. Anderson R., King A. D., Innis B. L. 1992; Correlation of the E protein binding with cell susceptibility to dengue 4 virus infection. J Gen Virol 73:2155–2159 [CrossRef]
    [Google Scholar]
  3. Bartenschlager R., Miller S. 2008; Molecular aspects of Dengue virus replication. Future Microbiol 3:155–165 [CrossRef]
    [Google Scholar]
  4. Blok J., Gibbs A. J., McWilliam S. M., Vitarana U. T. 1991; NS1 gene sequences from eight dengue-2 viruses and their evolutionary relationships with other dengue-2 viruses. Arch Virol 118:209–223 [CrossRef]
    [Google Scholar]
  5. Chen Y., Maguire T., Marks R. M. 1996; Demonstration of binding of dengue virus envelope protein to target cells. J Virol 70:8765–8772
    [Google Scholar]
  6. Chungue E., Cassar O., Drouet M. T. 1995; Molecular epidemiology of dengue-1 and dengue-4 viruses. J Gen Virol 76:1877–1884 [CrossRef]
    [Google Scholar]
  7. Deubel V., Nogueira R. M., Drouet M. T., Zeller H., Reynes J. M., Ha D. Q. 1993; Direct sequencing of genomic cDNA fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses. Arch Virol 129:197–210 [CrossRef]
    [Google Scholar]
  8. Domingo C., Palacios G., Jabado O., Reyes N., Niedrig M., Gascón J., Cabrerizo M., Lipkin W. I., Tenorio A. 2006; Use of short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India. J Clin Microbiol 44:1519–1529 [CrossRef]
    [Google Scholar]
  9. Gibbons R. V., Vaughn D. W. 2002; Dengue: an escalating problem. BMJ 324:1563–1566 [CrossRef]
    [Google Scholar]
  10. Goncalvez A. P., Escalante A. A., Pujol F. H., Ludert J. E., Tovar D., Salas R. A., Liprandi F. 2002; Diversity and evolution of the envelope gene of dengue virus type 1. Virology 303:110–119 [CrossRef]
    [Google Scholar]
  11. Gubler D. J. 1998a; The global pandemic of dengue/dengue hemorrhagic fever: current status and prospects for the future. Ann Acad Med Singapore 27:227–234
    [Google Scholar]
  12. Gubler D. J. 1998b; Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11:480–496
    [Google Scholar]
  13. Gubler D. J., Clark G. G. 1995; Dengue/dengue hemorrhagic fever: the emergence of a global health problem. Emerg Infect Dis 1:55–57 [CrossRef]
    [Google Scholar]
  14. Halstead S. B. 1988; Pathogenesis of dengue: challenges to molecular biology. Science 239:476–481 [CrossRef]
    [Google Scholar]
  15. Heinz F. X., Roehrig J. T. 1990; Flaviviruses. In Immunochemistry of Viruses Volume II The Basis of Serodiagnosis and Vaccines pp 289–305Edited by Regenmortel M. H. V., Neurath A. R. Amsterdam: Elsevier Science Publishers BV; (Biomedical Division
    [Google Scholar]
  16. Igarashi A., Fujita N., Okuno Y. 1982; Isolation of dengue viruses from patients with dengue haemorrhagic fever (DHF) and those with fever of unknown origin (FUO). in Jakarta, Indonesia, in the years of 1981 and 1982 ICMR Annals Kobe University 2, 7–17 (in Japanese)
    [Google Scholar]
  17. Kong Y. Y., Thay C. H., Tin T. C., Devi S. 2006; Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. J Virol Methods 138:123–130 [CrossRef]
    [Google Scholar]
  18. Kurane I., Ennis F. A. 1997; Immunopathogenesis of dengue virus infections. In Dengue and Dengue Hemorrhagic Fever pp 273–290Edited by Gubler D. J., Kuno G. Wallingford: CAB International;
    [Google Scholar]
  19. Laille M., Rochie C. 2004; Comparison of dengue-1 virus envelope glycoprotein gene sequences from French Polynesia. Am J Trop Med Hyg 71:478–484
    [Google Scholar]
  20. Lam S. K., Devi S., Pang T. 1987; Detection of specific IgM in dengue infections. Southeast Asian J Trop Med Public Health 18:532–538
    [Google Scholar]
  21. Lanciotti R. S., Lewis J. G., Gubler D. J., Trent D. W. 1994; Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 75:65–75 [CrossRef]
    [Google Scholar]
  22. Lee E., Weir C. W., Dalgarno L. 1997; Changes in the dengue virus major envelope protein on passaging and their localization on the three-dimensional structure of the protein. Virology 232:281–290 [CrossRef]
    [Google Scholar]
  23. Leitmeyer K. C., Vaughn D. W., Watts D. M., Salas R., Villalobos de Chacon I., Ramos C., Rico-Hesse R. 1999; Dengue virus structural difference that correlate with pathogenesis. J Virol 73:4738–4747
    [Google Scholar]
  24. Lewis J. A., Chang G. J., Lanciotti R. S., Kinney R. M., Mayer L. W., Trent D. W. 1993; Phylogenetic relationships of dengue-2 viruses. Virology 197:216–224 [CrossRef]
    [Google Scholar]
  25. Libraty D. H., Young P. R., Pickering D., Endy T. P., Kalayanarooj S., Green S., Vaughn D. W., Nisalak A., Ennis F. A., Rothman A. L. 2002; High circulating levels of dengue virus non-structural protein NS1 early in dengue illness correlate with the development of dengue haemorrhagic fever. J Infect Dis 186:1165–1168 [CrossRef]
    [Google Scholar]
  26. Lindenbach B. D., Rice C. M. 2001; Flaviviridae : the viruses and their replication. In Fields Virology , 4th edn. pp 991–1041Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  27. Monath T. P. 1994; Dengue: the risk to developed and developing countries. Proc Natl Acad Sci U S A 91:2395–2400 [CrossRef]
    [Google Scholar]
  28. Monath T. P., Heinz F. X. 1996; Flaviviruses, In Fields Virology . , 3rd edn. pp 961–1034Edited by Fields B. N., Knipe D. M., Howley. Philadelphia, PA: Lippincott-Raven Publishers;
  29. Ong S. H., Yip J. T., Chen Y. L., Liu W., Harun S., Lystiyaningsih E., Heriyanto B., Beckett C. G., Mitchell W. P. other authors 2008; Periodic re-emergence of endemic strains with strong epidemic potential—a proposed explanation for the 2004 Indonesian dengue epidemic. Infect Genet Evol 8:191–204 [CrossRef]
    [Google Scholar]
  30. Osman O., Fong M. Y., Devi S. 2007; A preliminary study of dengue infection in Brunei. Jpn J Infect Dis 60:205–208
    [Google Scholar]
  31. Rico-Hesse R. 1990; Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology 174:479–493 [CrossRef]
    [Google Scholar]
  32. Rico-Hesse R., Harrison L. M., Salas R. A. 1997; Origin of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology 230:244–251 [CrossRef]
    [Google Scholar]
  33. Rosen L. 1977; The emperor's new clothes revisited, or reflections on the pathogenesis of dengue hemorrhagic fever. Am J Trop Med Hyg 26:337–343
    [Google Scholar]
  34. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [CrossRef]
    [Google Scholar]
  35. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  36. Tantracheewathorn T., Tantracheewathorn S. 2007; Risk factors of dengue shock syndrome in children. J Med Assoc Thai 90:272–277
    [Google Scholar]
  37. WHO 1997 Dengue Hemorrhagic Fever: Diagnosis, Treatment, Prevention and Control , 2nd edn. Geneva: World Health Organization;
    [Google Scholar]
  38. WHO 2002 Fact sheet No 117 April 2002 Geneva: World Health Organization;
    [Google Scholar]
  39. WHO 2006 Annual Report. WHO Collaborating Centre For Arbovirus Reference and Research (Dengue and Dengue hemorrhagic Fever) – Malaysia. Geneva: World Health Organization;
    [Google Scholar]
  40. Young P. R., Hilditch P. A., Bletchly C., Halloran W. 2000; An antigen capture enzyme-linked immunosorbent assay reveals high levels of dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38:1053–1057
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.005306-0
Loading
/content/journal/jgv/10.1099/vir.0.005306-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error