%0 Journal Article %A Ong, Swee Tin %A Yusoff, Khatijah %A Kho, Chiew Ling %A Abdullah, Janna Ong %A Tan, Wen Siang %T Mutagenesis of the nucleocapsid protein of Nipah virus involved in capsid assembly %D 2009 %J Journal of General Virology, %V 90 %N 2 %P 392-397 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.005710-0 %I Microbiology Society, %X The nucleocapsid protein of Nipah virus produced in Escherichia coli assembled into herringbone-like particles. The amino- and carboxy-termini of the N protein were shortened progressively to define the minimum contiguous sequence involved in capsid assembly. The first 29 aa residues of the N protein are dispensable for capsid formation. The 128 carboxy-terminal residues do not play a role in the assembly of the herringbone-like particles. A region with amino acid residues 30–32 plays a crucial role in the formation of the capsid particle. Deletion of any of the four conserved hydrophobic regions in the N protein impaired capsid formation. Replacement of the central conserved regions with the respective sequences from the Newcastle disease virus restored capsid formation. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.005710-0