RT Journal Article SR Electronic(1) A1 Ong, Swee Tin A1 Yusoff, Khatijah A1 Kho, Chiew Ling A1 Abdullah, Janna Ong A1 Tan, Wen SiangYR 2009 T1 Mutagenesis of the nucleocapsid protein of Nipah virus involved in capsid assembly JF Journal of General Virology, VO 90 IS 2 SP 392 OP 397 DO https://doi.org/10.1099/vir.0.005710-0 PB Microbiology Society, SN 1465-2099, AB The nucleocapsid protein of Nipah virus produced in Escherichia coli assembled into herringbone-like particles. The amino- and carboxy-termini of the N protein were shortened progressively to define the minimum contiguous sequence involved in capsid assembly. The first 29 aa residues of the N protein are dispensable for capsid formation. The 128 carboxy-terminal residues do not play a role in the assembly of the herringbone-like particles. A region with amino acid residues 30–32 plays a crucial role in the formation of the capsid particle. Deletion of any of the four conserved hydrophobic regions in the N protein impaired capsid formation. Replacement of the central conserved regions with the respective sequences from the Newcastle disease virus restored capsid formation., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.005710-0